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Updated: May 23, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Nucleotide sequence of a chickpea chlorotic stunt virus relative that infects pea and faba bean in China
Cui-Ji Zhou1, Hai-Ying Xiang, Tao Zhuo
1State Key Laboratory for Agrobiotechnology and Department of Plant Pathology, China Agricultural University, Beijing 100193, China. mtracy@126.com
Abstract:
We determined the genome sequence of a new polerovirus that infects field pea and faba bean in China. Its entire nucleotide sequence (6021 nt) was most closely related (83.3% identity) to that of an Ethiopian isolate of chickpea chlorotic stunt virus (CpCSV-Eth). With the exception of the coat protein (encoded by ORF3), amino acid sequence identities of all gene products of this virus to those of CpCSV-Eth and other poleroviruses were <90%. This suggests that it is a new member of the genus Polerovirus, and the name pea mild chlorosis virus is proposed.
