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Related Experiment Videos

Posttranslational gastrin processing depends on tumor morphology.

C V Godellas1, W R Gower, P J Fabri

  • 1Department of Surgery, University of South Florida, Tampa 33612.

Surgery
|December 1, 1990
PubMed
Summary
This summary is machine-generated.

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Extracellular matrices influence cell shape, affecting posttranslational processing. Tumor cells grown as monolayers showed increased gastrin sulfation, while spheroid cultures maintained original levels.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Tumor Biology

Background:

  • Extracellular matrices (ECM) are known to influence cell morphology.
  • Altered cell morphology can affect gene transcription and translation.
  • The impact of ECM on posttranslational processing remains largely uncharacterized.

Purpose of the Study:

  • To investigate the effect of various substrates on posttranslational processing in gastrin-producing tumor (GT) cells.
  • To use tyrosine-O-sulfation as a marker to assess gastrin processing.
  • To correlate cell morphology (monolayer vs. spheroid) with gastrin production and sulfation.

Main Methods:

  • GT cells were cultured on different substrates (plastic, dried films, porous membranes, hydrated gels) to induce distinct morphologies.

Related Experiment Videos

  • Culture media were collected at multiple time points (days 4, 7, 28).
  • Specific antibodies were used to quantify total and nonsulfated biologically active gastrin (IRG).

    • Main Results:

    • Cells cultured on plastic and dried films formed monolayers and exhibited increased sulfation over time, but decreased IRG production.
    • Cells cultured on porous membranes and hydrated gels formed spheroids, maintaining constant sulfation levels and showing decreased IRG production (except on Matrigel gel).
    • Spheroid cultures preserved the sulfation level of the original tumor, unlike monolayer cultures.

    Conclusions:

    • ECM composition and resulting cell morphology significantly impact posttranslational processing of gastrin.
    • Monolayer cultures lead to increased sulfation, whereas spheroid cultures maintain basal sulfation levels.
    • Cellular microenvironment modulation alters cell morphology, subsequently affecting gastrin processing.