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A Decentralized (Ex Vivo) Murine Bladder Model with the Detrusor Muscle Removed for Direct Access to the Suburothelium during Bladder Filling
Published on: November 28, 2019
The validation of a functional, isolated pig bladder model for physiological experimentation.
Brian A Parsons1, Marcus J Drake, Andrew Gammie
1Southmead Hospital, Bristol Urological Institute Bristol, UK.
Frontiers in Pharmacology
|April 6, 2012
Summary
Researchers validated an isolated pig bladder model for studying bladder physiology. This preparation remains viable in vitro, responds to drug administration, and allows correlation of bladder wall movements with pressure changes.
Area of Science:
- Integrative physiology
- Urology
- Organ preparation models
Background:
- Studying bladder physiology requires whole organ preparations.
- Validating reliable animal models is crucial for physiological research.
Purpose of the Study:
- To validate an isolated large animal (pig) bladder preparation for studying integrative physiology.
- To assess the viability and responsiveness of the isolated bladder model.
Main Methods:
- Isolated pig bladders were arterially perfused in vitro.
- Drug administration via arterial and intravesical routes, pressure recording, and surface micromotion filming were employed.
- Bladder viability was assessed via histology and pH monitoring.
Main Results:
- The preparation remained viable for up to 8 hours with no structural deterioration.
- Arterial drug administration showed dose-dependent responses.
- Surface micromotions correlated variably with intravesical pressure fluctuations.
Conclusions:
- The isolated pig bladder is a valid model for integrative bladder physiology studies.
- The model allows for studying drug responses and correlating bladder wall movement with pressure.
- This preparation offers a robust platform for urological research.
