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Published on: March 16, 2016
A study on neuroinflammatory marker in brain areas of okadaic acid (ICV) induced memory impaired rats
Pradeep Kumar Kamat1, Santoshkumar Tota, Shivika Rai
1Division of Pharmacology, Central Drug Research Institute, Lucknow (U.P.), India.
Aims:
The aim of the present study is to investigate the status of proinflammatory cytokine in the brain of intracerebroventricular (i.c.v.) okadaic acid (OKA) induced memory impaired rat.
Main Methods:
OKA (200 ng) intracerebroventricular (i.c.v.) was administered in rats. Memory was assessed by Morris water maze test. Biochemical marker of neuroinflammation (TNF-α, IL-β), total nitrite, mRNA (RT PCR) and protein expression (WB) of iNOS and nNOS were estimated in rat brain areas.
Key Findings:
OKA caused memory-impairment in rats with increased expression of proinflammatory cytokine TNF-α and IL-1β and total nitrite in brain regions hippocampus and cortex. The expression of mRNA and protein of iNOS was increased while; the expressions were decreased in case of nNOS. Pretreatment with antidementic drugs donepezil (5 mg/kg, p.o.) and memantine (10 mg/kg, p.o) for 13 days protected i.c.v. OKA induced memory impairment and changes in level of TNF-α, IL-β, total nitrite and expressions of iNOS and nNOS in OKA treated rat.
Significance:
This study suggests that neuroinflammation may play a vital role in OKA induced memory impairment.
