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Electrospray-differential mobility analysis of bionanoparticles.

Suvajyoti Guha1, Mingdong Li, Michael J Tarlov

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Area of Science:

  • Analytical Chemistry
  • Nanotechnology
  • Biophysics

Background:

  • Electrospray (ES) and differential mobility analysis (DMA) are established techniques.
  • Combining ES and DMA (ES-DMA) offers a powerful method for particle characterization in liquids.
  • ES-DMA complements techniques like electrospray-mass spectrometry (ES-MS) by measuring particle size instead of mass.

Purpose of the Study:

  • To review the electrospray-differential mobility analysis (ES-DMA) technique.
  • To highlight the application of ES-DMA in characterizing bionanoparticles.
  • To discuss the versatility and growing use of ES-DMA for liquid-phase analysis.

Main Methods:

  • Aerosolization of bionanoparticles using electrospray.
  • Measurement of electrical mobility using differential mobility analysis.
  • Characterization of particle size distributions at ambient conditions.

Main Results:

  • ES-DMA can characterize a wide range of bionanoparticles, from ~3 nm to several hundred nanometers.
  • The technique provides multimodal size distributions rapidly (in minutes).
  • Recent applications include the characterization of polymers, proteins, viruses, bacteriophages, and nanoparticle-biomolecule conjugates.

Conclusions:

  • ES-DMA is a versatile and effective technique for bionanoparticle characterization.
  • The combination of ES and DMA is increasingly utilized for analyzing particles in liquid environments.
  • ES-DMA offers rapid, size-based characterization of diverse nanomaterials and biomolecules.