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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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Related Experiment Video

Updated: May 23, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

The spliceosome: a flexible, reversible macromolecular machine.

Aaron A Hoskins1, Melissa J Moore

  • 1Department of Biochemistry, University of Wisconsin-Madison, WI 53706, USA. ahoskins@wisc.edu

Trends in Biochemical Sciences
|April 7, 2012
PubMed
Summary

Recent advances enable detailed study of the spliceosome, a complex cellular machine. New methods reveal its dynamic nature and how pre-mRNA influences its function.

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Last Updated: May 23, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • The spliceosome is a highly complex molecular machine responsible for RNA splicing in eukaryotic cells.
  • Its intricate assembly and disassembly pathways and large number of components make mechanistic studies challenging.

Purpose of the Study:

  • To explore recent technological advancements facilitating the detailed kinetic and mechanistic analysis of spliceosome function.
  • To understand the dynamic and substrate-influenced nature of the spliceosome.

Main Methods:

  • Genome-wide analyses of splicing.
  • In vivo creation of an orthogonal spliceosome.
  • Purification of active spliceosomes.
  • Single-molecule observations in vitro.

Main Results:

  • New experimental approaches provide unprecedented insight into spliceosome's inner workings.
  • Evidence suggests the spliceosome is a dynamic and malleable machine.
  • The pre-mRNA substrate significantly influences spliceosome activity.

Conclusions:

  • Technological innovations are revolutionizing the study of spliceosome dynamics and mechanism.
  • A revised understanding of the spliceosome as a dynamic, substrate-responsive machine is emerging.