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Related Experiment Video

Updated: May 23, 2026

Establishment of an Embryo Implantation Model In Vitro

Establishment of an Embryo Implantation Model In Vitro

Published on: June 21, 2024

Synchronization between embryo development and endometrium is a contributing factor for rescue ICSI outcome.

Li Ming¹, Ping Liu, Jie Qiao

¹Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, People’s Republic of China.

Reproductive Biomedicine Online

|April 7, 2012

Summary

Clinical pregnancy rates after rescue intracytoplasmic sperm injection (ICSI) are poor. Cryopreserving embryos from rescue ICSI and using them in frozen-thawed cycles improves synchronization and significantly increases pregnancy and implantation rates.

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Area of Science:

Reproductive Medicine
Embryology
Infertility Treatment

Background:

Rescue intracytoplasmic sperm injection (ICSI) is performed for fertilization failure in conventional in vitro fertilization (IVF) cycles.
Current outcomes for rescue ICSI are unsatisfactory, with low clinical pregnancy rates.
Potential causes include oocyte aging and asynchrony between embryo development and endometrial receptivity.

Purpose of the Study:

To investigate the impact of embryo developmental stage and endometrial secretory pattern synchronization on rescue ICSI outcomes.
To compare the efficacy of fresh embryo transfer versus frozen-thawed embryo transfer following rescue ICSI.

Main Methods:

Retrospective analysis of 534 fresh rescue ICSI cycles and 64 frozen-thawed transfer cycles.
Cycles were divided into groups: fresh embryo transfer (FET), FET with cryopreservation of surplus embryos, and frozen-thawed transfer (FT).
Comparison focused on FET with cryopreservation (Group II) versus FT (Group III).

Main Results:

Group III (frozen-thawed transfer) demonstrated significantly higher clinical pregnancy rates (29.69%) compared to Group II (10.81%).
Implantation rates were also significantly higher in Group III (13.33%) versus Group II (5.13%).
No significant difference in maternal age was observed between the compared groups.

Conclusions:

Synchronization between embryo development and the endometrium is a critical factor for successful rescue ICSI outcomes.
Cryopreservation of embryos derived from rescue ICSI cycles is recommended.
Subsequent frozen-thawed embryo transfer cycles offer improved pregnancy and implantation rates compared to fresh transfers.