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Updated: May 23, 2026

In Vitro Polymerization of F-actin on Early Endosomes
Published on: August 28, 2017
Vesicle formation within endosomes: An ESCRT marks the spot
Jonathan R Mayers1, Anjon Audhya
1Department of Biomolecular Chemistry; University of Wisconsin-Madison Medical School; Madison, WI USA.
Abstract:
Vesicle-mediated cargo transport within the endomembrane system requires precise coordination between adaptor molecules, which recognize sorting signals on substrates, and factors that promote changes in membrane architecture. At endosomal compartments, a set of protein complexes collectively known as the ESCRT machinery sequesters transmembrane cargoes that harbor a ubiquitin modification and packages them into vesicles that bud into the endosome lumen. Several models have been postulated to describe this process. However, consensus in the field remains elusive. Here, we discuss recent findings regarding the structure and function of the ESCRT machinery, highlighting specific roles for ESCRT-0 and ESCRT-III in regulating cargo selection and vesicle formation.
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