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Related Experiment Video

Updated: May 23, 2026

In Vitro Polymerization of F-actin on Early Endosomes

In Vitro Polymerization of F-actin on Early Endosomes

Published on: August 28, 2017

Vesicle formation within endosomes: An ESCRT marks the spot.

Jonathan R Mayers¹, Anjon Audhya

¹Department of Biomolecular Chemistry; University of Wisconsin-Madison Medical School; Madison, WI USA.

Communicative & Integrative Biology

|April 7, 2012

Summary

The ESCRT machinery sorts ubiquitinated proteins into vesicles within endosomes. This review highlights ESCRT-0 and ESCRT-III roles in cargo selection and vesicle formation, clarifying endosomal transport mechanisms.

Keywords:

intralumenal vesicle membrane curvature membrane scission membrane trafficking multivesicular endosome phosphatidylinositol 3-phosphate ubiquitin

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The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Published on: February 12, 2022

Area of Science:

Cell Biology
Molecular Biology
Membrane Trafficking

Background:

Vesicle-mediated transport is crucial for the endomembrane system.
Adaptor molecules and membrane-shaping factors coordinate this process.
The ESCRT machinery (Endosomal Sorting Complexes Required for Transport) is key for sequestering ubiquitinated transmembrane cargo in endosomes.

Purpose of the Study:

To review recent findings on the structure and function of the ESCRT machinery.
To highlight the specific roles of ESCRT-0 and ESCRT-III in cargo selection and vesicle formation.
To address the lack of consensus regarding ESCRT-mediated endosomal transport models.

Main Methods:

Literature review of recent structural and functional studies on the ESCRT machinery.
Analysis of experimental data concerning ESCRT-0 and ESCRT-III interactions and mechanisms.
Synthesis of current models and identification of areas lacking consensus.

Main Results:

Recent findings provide new insights into the structural organization of ESCRT complexes.
Specific functions of ESCRT-0 in cargo recognition and ESCRT-III in membrane remodeling have been elucidated.
These advances contribute to a better understanding of how ubiquitinated cargo is selected and packaged into intraluminal vesicles.

Conclusions:

The ESCRT machinery, particularly ESCRT-0 and ESCRT-III, plays critical roles in endosomal cargo sorting and vesicle formation.
Further research is needed to fully resolve the mechanistic details and achieve consensus on ESCRT pathway regulation.
Understanding ESCRT function is vital for comprehending endosomal transport and its implications in cellular processes and disease.