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Updated: May 23, 2026
In vitro Organoid Culture of Primary Mouse Colon Tumors
Published on: May 17, 2013
[Biological behaviour of colon cancer cells transfected with C-erbB2 shRNA plasmid]
Dong-Li Zhao1, Cheng-Xue Dang, Yan-Xia Sui
1Department of Oncology, First Affiliated Hospital, Medical College of Xi'an Jiaotong University, Xi'an 710061, China. zhaodongli68@163.com
Aim:
To study the changes of the cell growth, cell cycle distribution and cell apoptosis of colon cancer cell, HT-29, when C-erbB2 gene was knockdown by shRNA against C-erbB2.
Methods:
Cell growth, cell cycle distribution and cell apoptosis were compared among three groups including plasmid experimental group(PEG), transfected reagent control group(TRCG)and negative plasmid control group(NPCG). Cell growth was measured by MTT assay. Cell cycle distribution and cell apoptosis were detected by flow cytometry.
Results:
The inhibition rate of cell growth of PEG, TRCG and NPCG were 39.65%, 7.23% and 8.05% respectively. The cell growth was significantly inhibited in PEG(P<0.01). The cells of G0/G1 phase were 74.93%, 67.19%, 68.05% respectively in PEG, TRCG and NPCG. The cells of G0/G1 phase in PEG were significantly more than those in TRCG and NPCG(P<0.05).While the cells of S phase were 7.81%, 14.02%, 13.70% in PEG, TRCG and NPCG respectively. The cells of S phase in PEG were significantly less than those in TRCG or NPCG(P<0.05).The cell apoptosis rate were 19.21%, 3.13%, 4.08% in PEG, TRCG and NPCG respectively. The cell apoptosis rate in PEG was significantly higher than those in TRCG or NPCG(P<0.01).
Conclusion:
Cell growth was inhibited by shRNA against C-erbB2 gene. Cell cycle was blocked in G0/G1 phase and apoptosis was induced by C-erbB2 shRNA. This indicates C-erbB2 gene plays important roles in the carcinogenesis and development of colon cancer.
Insights
Knocking down the C-erbB2 gene with shRNA significantly inhibited colon cancer cell growth. This C-erbB2 gene knockdown also induced cell apoptosis and cell cycle arrest in the G0/G1 phase.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Context:
- Colon cancer is a significant global health concern.
- The C-erbB2 gene is implicated in various cancers, including colon cancer.
- Understanding C-erbB2's role is crucial for developing targeted therapies.
Purpose:
- To investigate the effect of C-erbB2 gene knockdown on HT-29 colon cancer cells.
- To analyze changes in cell growth, cell cycle distribution, and apoptosis following C-erbB2 inhibition.
Summary:
- Short hairpin RNA (shRNA) targeting the C-erbB2 gene was used to knockdown its expression in HT-29 colon cancer cells.
- Cell growth was significantly inhibited (39.65% vs. control), with cells arrested in the G0/G1 phase and increased apoptosis (19.21% vs. control).
- These findings demonstrate C-erbB2's critical role in colon cancer progression.
Impact:
- Provides evidence for C-erbB2 as a potential therapeutic target in colon cancer treatment.
- Highlights the utility of shRNA-mediated gene silencing in cancer research.
- Contributes to the understanding of molecular mechanisms underlying colon carcinogenesis.
