Ribosome-inactivating proteins: current status and biomedical applications
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Updated: May 23, 2026

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
Published on: April 21, 2016
Munish Puri1, Inderdeep Kaur, Matthew A Perugini
1Centre for Biotechnology and Interdisciplinary Sciences (BioDeakin), Institute for Technology Research and Innovation, Deakin University, VIC 3217, Australia. munish.puri@deakin.edu.au
Abstract:
Ribosome-inactivating proteins (RIPs) are mainly present in plants and function to inhibit protein synthesis through the removal of adenine residues from eukaryotic ribosomal RNA (rRNA). They are broadly classified into two groups: type I and type II. Type I RIPs are a diverse family of proteins comprising a single polypeptide chain, whereas type II RIPs are heterodimeric glycoproteins comprising an A-chain (functionally equivalent to a type I RIP) linked via a disulphide bond to a B chain, mediating cell entry. In this review, we describe common type I and type II RIPs, their diverse biological functions, mechanism of cell entry, stability in plasma and antigenicity. We end with a discussion of promising applications for RIPs in biomedicine.
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