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Related Experiment Video

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Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

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On-line multiple component analysis for efficient quantitative bioprocess development.

Christian Dietzsch¹, Oliver Spadiut, Christoph Herwig

¹Vienna University of Technology, Institute of Chemical Engineering, Research Area Biochemical Engineering, Vienna, Austria.

Journal of Biotechnology

|April 10, 2012

Summary

This study introduces a novel on-line monitoring device for real-time bioprocess analysis. The system ensures high data quality, enabling accurate quantification and physiological insights for Pichia pastoris cultivations.

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PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

Area of Science:

Biotechnology
Process Analytical Technology (PAT)
Microbial Cultivation

Background:

Accurate on-line monitoring is crucial for rapid bioprocess quantification.
Real-time data quality assessment is essential for reliable quantitative information extraction.
Existing methods may lack the precision and automation required for comprehensive bioprocess analysis.

Purpose of the Study:

To characterize a novel on-line sampling and analysis device for bioprocess monitoring.
To evaluate the device's performance in real-time measurement of multiple components during Pichia pastoris cultivation.
To demonstrate the utility of high-quality on-line data for bioprocess understanding and control.

Main Methods:

Development and implementation of an automated photometric robot for on-line sampling and analysis.
Connection of the on-line device to a bioreactor for Pichia pastoris batch cultivations.
Concomitant measurement of six key components: glucose, glycerol, ethanol, acetate, phosphate, and ammonium.

Main Results:

On-line measurements showed no significant deviation compared to off-line samples, confirming data accuracy.
Calculated on-line rates and yields accurately reflected Pichia pastoris physiological responses.
Integration with off-line biomass data enabled detailed analysis of specific rates and cellular physiology.

Conclusions:

The novel on-line device provides high-quality, real-time data for bioprocess monitoring.
This technology is a valuable tool for Process Analytical Technology (PAT), enhancing bioprocess understanding.
Accurate on-line data facilitates precise rate and yield calculations, crucial for optimizing microbial cultivations.