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Updated: May 23, 2026

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

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Published on: August 10, 2009

KOMA: ELISA-microarray calibration and data analysis based on kinetic signal amplification.

Pavlo Holenya¹, Florian Heigwer, Stefan Wölfl

¹Institut für Pharmazie und Molekulare Biotechnologie, Ruperto-Carola University of Heidelberg, Heidelberg, Germany.

Journal of Immunological Methods

|April 10, 2012

Summary

This study introduces KOMA, open-source software for analyzing quantitative microarray data. It enhances protein analysis precision and quantification range using kinetic detection of enzymatic signals.

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Area of Science:

Biotechnology
Bioinformatics
Analytical Chemistry

Background:

Antibody microarrays with enzyme-linked immunosorbent technology enable high-throughput, quantitative protein analysis.
Kinetic detection methods can improve the precision and quantification range of microarray measurements.

Purpose of the Study:

To present the open-source software Kinetic Operating Microarray Analyzer (KOMA).
To enable calibration and high-throughput analysis of quantitative microarray data using time-resolved kinetic detection.
To provide a tool for analyzing data from other enzymatic assays with time-resolved readouts.

Main Methods:

Development and application of the Kinetic Operating Microarray Analyzer (KOMA) software.
Utilizing a time-resolved kinetic detection protocol for enzymatic signals in antibody microarrays.
Employing enzyme-linked immunosorbent technology for quantitative protein analysis.

Main Results:

KOMA facilitates calibration and high-throughput analysis of quantitative microarray data.
The software improves precision and quantification range through kinetic detection.
KOMA is applicable to various analytical assays employing enzymatic signal amplification.

Conclusions:

KOMA is a valuable open-source tool for analyzing quantitative microarray data with kinetic detection.
The software enhances the capabilities of high-throughput protein analysis.
KOMA supports broader quantification ranges in enzymatic assays through time-resolved data recording.