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Related Experiment Video

Updated: May 23, 2026

Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae
12:13

Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae

Published on: April 5, 2015

6-azauracil sensitivity assay for yeast.

William P Tansey1

  • 1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

CSH Protocols
|April 10, 2012
PubMed
Summary

Yeast treated with 6-Azauracil (6AU) shows reduced GTP levels, which inhibits growth in specific mutant strains. This 6AU sensitivity serves as a valuable assay for identifying mutations impacting transcriptional elongation in yeast.

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Area of Science:

  • * Molecular Biology
  • * Yeast Genetics

Background:

  • * 6-Azauracil (6AU) treatment reduces intracellular guanosine triphosphate (GTP) levels in yeast.
  • * Reduced GTP levels are not lethal but can impede yeast growth when combined with mutations affecting transcriptional elongation.

Purpose of the Study:

  • * To establish 6-Azauracil sensitivity as a method for identifying yeast mutants with defects in transcriptional elongation.
  • * To provide a straightforward assay for studying transcriptional elongation in yeast.

Main Methods:

  • * Yeast cultures were grown to saturation.
  • * Yeast cell counts were performed.
  • * Serial dilutions of yeast were spotted onto both standard (CSM) and 6-Azauracil-containing (CSM + 6AU) plates.

Main Results:

  • * Yeast strains with mutations affecting transcriptional elongation exhibited sensitivity to 6AU.
  • * The assay allowed for the identification of strains with impaired transcriptional elongation based on growth inhibition on 6AU plates.

Conclusions:

  • * 6-Azauracil sensitivity is a viable and practical assay for detecting defects in yeast transcriptional elongation.
  • * This method can be utilized as a screening tool in yeast genetics research.

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