Related Experiment Video
Updated: May 23, 2026
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
Tumor suppressor miR-22 suppresses lung cancer cell progression through post-transcriptional regulation of ErbB3
Bo Ling1, Gui-Xue Wang, Guang Long
1Key Laboratory of Biological Science and Technology, Chongqing University, Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400044, China.
Background:
Development of efficient therapies of lung cancer and deep understanding of their anti-tumor mechanism are very important. The aim of the present study is to investigate the therapeutic effect of microRNA-22 (miR-22) on lung cancer using in vitro and in vivo methods.
Methods:
Expression level of miR-22 in lung cancer specimens and relative normal tissues was detected by microRNA-specific quantitative real-time PCR (Q-PCR). Invasion assay, cell counting kit-8 assay, and Annexin V/7-AAD analysis were performed to test the invasion and proliferation of lung cancer cell after transfection. The effect of miR-22 on lung cancer in vivo was validated by murine xenograft model.
Results:
Q-PCR detection of miR-22 in clinical samples showed that the relative expression level of miR-22 in lung cancer tissues and lung cancer cell lines was lower than that in normal tissues. Transfection of miR-22 expression plasmids could significantly inhibit the increased cell numbers and invasion of A549 and H1299 lung cancer cell lines. Furthermore, miR-22 was demonstrated to inhibit the expression of ErbB3 through post-transcriptional regulation via binding to ErbB3 3'-UTR. Co-transfection of ErbB3 expression plasmid could promote the proliferation and invasion of A549 and H1299. In vivo experiments using nude mice demonstrated that over-expression of miR-22 could significantly decrease the volume and weight of tumors.
Conclusions:
miR-22 exhibited excellent anti-lung cancer activity in vitro and in vivo, and post-transcriptional regulation of ErbB3 might be a potential mechanism.
Insights
MicroRNA-22 (miR-22) shows significant anti-lung cancer activity by inhibiting tumor growth and invasion. This study reveals miR-22
Area of Science:
- Oncology
- Molecular Biology
- Gene Regulation
Background:
- Lung cancer necessitates novel therapeutic strategies.
- Understanding anti-tumor mechanisms is crucial for treatment development.
Purpose of the Study:
- To investigate the therapeutic potential of microRNA-22 (miR-22) in lung cancer.
- To elucidate the anti-tumor mechanisms of miR-22.
Main Methods:
- Quantitative real-time PCR (Q-PCR) to assess miR-22 expression in clinical samples.
- In vitro assays (invasion, proliferation, apoptosis) and in vivo murine xenograft models to evaluate miR-22's efficacy.
- Analysis of miR-22's interaction with ErbB3 via 3'-UTR binding.
Main Results:
- Reduced miR-22 expression observed in lung cancer tissues and cell lines.
- miR-22 transfection significantly inhibited lung cancer cell proliferation and invasion.
- miR-22 targets ErbB3, suppressing its expression and consequently reducing tumor growth in vivo.
- Overexpression of ErbB3 reversed the inhibitory effects of miR-22.
Conclusions:
- miR-22 demonstrates potent anti-lung cancer activity both in vitro and in vivo.
- Post-transcriptional regulation of ErbB3 by miR-22 is a key mechanism underlying its anti-tumor effects.
- miR-22 represents a promising therapeutic target for lung cancer treatment.
Related Concept Videos
01:22MicroRNAs
01:22MicroRNAs
02:23Abnormal Proliferation
01:20The Retinoblastoma Gene
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
01:12Loss of Tumor Suppressor Gene Functions
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
01:12Loss of Tumor Suppressor Gene Functions
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...