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Proliferative and morphologic characterization of buccal mucosal fibroblasts in areca nut chewers: a cell culture
Deepu George Mathew1, K Skariah Skariah, Kannan Ranganathan
1Department of Oral and Maxillofacial Pathology, Ragas Dental College and Hospital, Chennai, India. deepugeorgemathew@gmail.com
Objective:
To isolate, culture and characterize fibroblasts from the buccal mucosa of areca nut chewers with and without oral submucous fibrosis (OSF).
Materials And Methods:
Primary fibroblast cultures were established by the collagenase disaggregation technique and the phenotypic and growth characteristics were studied.
Results:
Cells cultured from OSF showed a statistically significant increase in both the post-mitotic fibroblast subpopulation and the population doubling time when compared with controls.
Conclusion:
There was a significant increase in the pro-fibrotic, post-mitotic subpopulation of fibroblasts in areca nut chewers with OSF.
