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Characterization of D-lactate dehydrogenase producing D-3-phenyllactic acid from Pediococcus pentosaceus
Shuhuai Yu1, Houyi Jiang, Bo Jiang
1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.
Abstract:
D-Lactate dehydrogenase (D-LDH) from Pediococcus pentosaceus ATCC 25745 was found to produce D-3-phenyllactic acid from phenylpyruvate. The optimum pH and temperature for enzyme activity were pH 5.5 and 45 °C. The Michaelis-Menten constant (K(m)), turnover number (k(cat)), and catalytic efficiency (k(cat)/K(m)) values for the substrate phenylpyruvate were estimated to be 1.73 mmol/L, 173 s(-1), and 100 (mmol/L)(-1) s(-1) respectively.
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