Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

How Contaminated Is the Surgical Field in Reverse Total Shoulder Arthroplasty? A Preliminary Quantitative Intraoperative Microbiological Study.

Journal of clinical medicine·2026
Same author

Risk Stratification for Late-Onset CMV Infection Following Prophylaxis With Letermovir in Allogeneic Stem Cell Transplant Recipients: Unraveling the Potential Role of CMV-Specific Cell-Mediated Immunity.

Transplant infectious disease : an official journal of the Transplantation Society·2026
Same author

Evaluation of cefepime/enmetazobactam gradient strip and disk diffusion test for CTX-M-producing Enterobacterales.

Journal of global antimicrobial resistance·2026
Same author

Rapid AST in practice - a workflow analysis of the QuickMIC<sup>®</sup> rapid AST system at multiple clinical laboratories in Europe.

Frontiers in cellular and infection microbiology·2026
Same author

Long-term neurological outcomes after extracorporeal cardiopulmonary resuscitation for refractory cardiac arrest: a 14-year single-centre cohort study.

Resuscitation·2026
Same author

In vivo emergence of cross-resistance to ceftazidime/avibactam and cefiderocol through selection of KPC variants in Klebsiella pneumoniae clinical isolates.

International journal of antimicrobial agents·2026

Related Experiment Video

Updated: May 23, 2026

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
12:57

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity

Published on: November 16, 2017

Alternative molecular tests for virological diagnosis.

Francesca Sidoti1, Massimiliano Bergallo, Cristina Costa

  • 1Department of Public Health and Microbiology, University Hospital San Giovanni Battista di Torino, University of Turin, Turin, Italy.

Molecular Biotechnology
|April 10, 2012
PubMed
Summary

Isothermal nucleic acid amplification techniques offer a simpler, faster, and more cost-effective alternative to conventional PCR for molecular diagnostics. These methods, including LAMP and NAATs, eliminate the need for complex equipment, enhancing accessibility in clinical settings.

More Related Videos

VirWaTest, A Point-of-Use Method for the Detection of Viruses in Water Samples
13:32

VirWaTest, A Point-of-Use Method for the Detection of Viruses in Water Samples

Published on: May 11, 2019

Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach
07:06

Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach

Published on: December 1, 2011

Related Experiment Videos

Last Updated: May 23, 2026

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
12:57

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity

Published on: November 16, 2017

VirWaTest, A Point-of-Use Method for the Detection of Viruses in Water Samples
13:32

VirWaTest, A Point-of-Use Method for the Detection of Viruses in Water Samples

Published on: May 11, 2019

Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach
07:06

Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach

Published on: December 1, 2011

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Clinical Diagnostics

Background:

  • Conventional nucleic acid amplification techniques (NAATs) like PCR are standard in clinical labs for rapid, sensitive, and specific diagnosis.
  • However, PCR requires specialized equipment for temperature cycling and analysis, limiting its accessibility.
  • Isothermal amplification methods present an attractive alternative by operating at a constant temperature.

Purpose of the Study:

  • To review and compare emerging isothermal nucleic acid amplification techniques with conventional NAATs.
  • To discuss the design criteria, amplification potential, and applications of these alternative molecular tests.
  • To highlight the advantages of isothermal methods for simplified and cost-effective molecular diagnostics.

Main Methods:

  • Review of isothermal amplification techniques: loop-mediated isothermal amplification (LAMP), nucleic acid sequence-based amplification (NASA), and helicase-dependent amplification (HDA).
  • Comparison of isothermal methods with conventional polymerase chain reaction (PCR) and real-time PCR.
  • Analysis of design criteria, amplification efficiency, and diagnostic applications.

Main Results:

  • Isothermal amplification techniques offer rapid reaction times and easy detection.
  • These methods simplify operation and reduce the need for sophisticated and costly equipment.
  • LAMP, NASA, and HDA show significant potential as accessible diagnostic tools.

Conclusions:

  • Isothermal nucleic acid amplification offers a viable and advantageous alternative to conventional NAATs for clinical diagnostics.
  • The simplicity and cost-effectiveness of isothermal methods can broaden the accessibility of molecular testing.
  • Further exploration of LAMP, NASA, and HDA is warranted for diverse diagnostic applications.