Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
Meiosis II01:57

Meiosis II

Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each containing...
Meiosis II02:02

Meiosis II

Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of Tau overexpression on DNA replication dynamics in centromeres of human neural progenitor cells.

iScience·2026
Same author

DNA2 and FANCM function in two distinctive pathways in disrupting TERRA R-loops and suppressing replication stress at ALT telomeres.

bioRxiv : the preprint server for biology·2025
Same author

Elucidation of the molecular mechanism of the breakage-fusion-bridge (BFB) cycle using a CRISPR-dCas9 cellular model.

Nucleic acids research·2024
Same author

Elucidation of the molecular mechanism of the breakage-fusion-bridge (BFB) cycle using a CRISPR-dCas9 cellular model.

bioRxiv : the preprint server for biology·2024
Same author

A local ATR-dependent checkpoint pathway is activated by a site-specific replication fork block in human cells.

eLife·2023
Same author

A local ATR-dependent checkpoint pathway is activated by a site-specific replication fork block in human cells.

bioRxiv : the preprint server for biology·2023

Related Experiment Video

Updated: May 23, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

G1/S phase synchronization using mimosine arrest.

Paul J Galgano, Carl L Schildkraut

    CSH Protocols
    |April 10, 2012
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a cell synchronization protocol using thymidine and the plant amino acid mimosine. This method effectively synchronizes cells at the G(1)/S phase boundary for further research.

    More Related Videos

    Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
    12:02

    Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

    Published on: June 6, 2017

    Laser Micro-Irradiation to Study DNA Recruitment During S Phase
    07:11

    Laser Micro-Irradiation to Study DNA Recruitment During S Phase

    Published on: April 16, 2021

    Related Experiment Videos

    Last Updated: May 23, 2026

    Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
    08:33

    Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

    Published on: December 5, 2017

    Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
    12:02

    Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

    Published on: June 6, 2017

    Laser Micro-Irradiation to Study DNA Recruitment During S Phase
    07:11

    Laser Micro-Irradiation to Study DNA Recruitment During S Phase

    Published on: April 16, 2021

    Area of Science:

    • Cell biology
    • Molecular biology
    • Biochemistry

    Background:

    • Cell cycle synchronization is crucial for studying cellular processes.
    • Existing methods may have limitations in efficiency or specificity.
    • The G(1)/S phase transition is a key regulatory point in the cell cycle.

    Purpose of the Study:

    • To present a reliable protocol for synchronizing cells at the G(1)/S phase.
    • To utilize the plant amino acid mimosine as a G(1)/S phase arresting agent.
    • To establish a method for controlled cell cycle progression.

    Main Methods:

    • Cells are initially treated with excess thymidine to induce G(1)/S arrest.
    • Thymidine is removed to allow synchronized progression through the S phase.
    • Mimosine is subsequently added to arrest cells at the G(1)/S border.
    • Removal of mimosine initiates synchronized entry into the S phase.

    Main Results:

    • The protocol effectively synchronizes a majority of cells at the G(1)/S phase.
    • Mimosine treatment provides a reliable arrest point at the G(1)/S border.
    • Upon mimosine removal, cells commence S phase entry within approximately one hour.

    Conclusions:

    • This protocol offers an effective method for G(1)/S phase cell synchronization.
    • The combination of thymidine and mimosine provides a robust two-step synchronization strategy.
    • This technique facilitates precise temporal control over cell cycle progression for experimental purposes.