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Related Concept Videos

SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termedĀ  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...

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Related Experiment Video

Updated: May 23, 2026

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
06:24

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Published on: April 21, 2019

Staining SDS-Polyacrylamide Gels with Silver Salts.

Joseph Sambrook, David W Russell

    CSH Protocols
    |April 10, 2012
    PubMed
    Summary

    Silver staining offers superior sensitivity for detecting polypeptides after SDS-polyacrylamide gel electrophoresis. This method is 100-1000 times more sensitive than Coomassie Brilliant Blue R-250 staining.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Analytical Chemistry

    Background:

    • SDS-polyacrylamide gel electrophoresis (SDS-PAGE) is a standard technique for separating polypeptides.
    • Traditional staining methods like Coomassie Brilliant Blue R-250 have limitations in sensitivity.
    • Highly sensitive detection methods are crucial for analyzing low-abundance proteins.

    Purpose of the Study:

    • To evaluate the sensitivity and applicability of silver staining for polypeptide detection.
    • To compare silver staining with Coomassie Brilliant Blue R-250 staining.
    • To establish a reliable protocol for sensitive protein visualization.

    Main Methods:

    • Separation of polypeptides using SDS-polyacrylamide gel electrophoresis.
    • Application of silver salt staining protocols.

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    A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

    Published on: April 20, 2018

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    Last Updated: May 23, 2026

    Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
    06:24

    Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

    Published on: April 21, 2019

    Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
    05:57

    Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining

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    A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
    10:27

    A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

    Published on: April 20, 2018

  • Comparison of staining intensity and detection limits with Coomassie Brilliant Blue R-250.

    • Main Results:

    • Silver staining demonstrated a sensitivity 100-1000 times greater than Coomassie Brilliant Blue R-250.
    • Detection of polypeptides down to 0.1-1.0 ng per band was achieved.
    • The method is effective for visualizing even trace amounts of proteins.

    Conclusions:

    • Silver staining is a highly sensitive and effective method for detecting polypeptides post-SDS-PAGE.
    • It significantly outperforms Coomassie Brilliant Blue R-250 in terms of sensitivity.
    • This technique is valuable for researchers needing to detect low-concentration proteins.