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Updated: May 23, 2026

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Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
Published on: April 21, 2019
Staining SDS-Polyacrylamide Gels with Silver Salts
CSH Protocols
|April 10, 2012
Abstract:
INTRODUCTIONA number of methods have been developed to stain polypeptides with silver salts after separation by SDS-polyacrylamide gel electrophoresis. It is somewhat more difficult to perform than staining with Coomassie Brilliant Blue R-250, but it is ~100-1000-fold more sensitive and is capable of detecting as little as 0.1-1.0 ng of polypeptide in a single band.
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