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Related Experiment Video

Updated: May 23, 2026

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery
08:38

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

Published on: October 4, 2018

Phosphoprotein Staining with the GelCode Phosphoprotein Staining Kit.

Wayne R Stochaj, Tom Berkelman, Nancy Laird

    CSH Protocols
    |April 10, 2012
    PubMed
    Summary
    This summary is machine-generated.

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    This study introduces a novel staining method for detecting phosphoproteins in gels. The technique utilizes a specific chemical reaction to visualize proteins with phosphate groups, achieving detection in the nanogram range.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Cellular Biology

    Background:

    • Protein phosphorylation is crucial for regulating cellular processes.
    • Accurate detection of phosphorylated proteins is essential for biological research.
    • Existing methods may have limitations in sensitivity or applicability.

    Purpose of the Study:

    • To present a new, sensitive method for detecting phosphoproteins in polyacrylamide gels.
    • To provide a detailed protocol for the GelCode phosphoprotein staining kit.
    • To establish the detection limits for specific phosphoproteins using this method.

    Main Methods:

    • Utilizes the GelCode phosphoprotein staining kit.
    • Involves hydrolysis of phosphoprotein phosphoester linkages with sodium hydroxide and calcium ions.

    More Related Videos

    An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
    07:45

    An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

    Published on: June 6, 2022

    Related Experiment Videos

    Last Updated: May 23, 2026

    Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery
    08:38

    Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

    Published on: October 4, 2018

    An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
    07:45

    An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

    Published on: June 6, 2022

  • Employs ammonium molybdate in nitric acid to form a detectable complex.
  • Stains the complex with Methyl Green for visualization.

    • Main Results:

    • Phosphoproteins are visualized as green to green-blue bands after staining and destaining.
    • The method achieves detection limits in the nanogram range.
    • Specific detection limits were determined for phosvitin (40-80 ng/band) and β-casein (80-160 ng/band).

    Conclusions:

    • The presented method offers a sensitive approach for detecting phosphoproteins.
    • This technique is suitable for analyzing phosphoproteins in minigel formats.
    • The protocol provides a valuable tool for researchers studying protein phosphorylation.