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Related Concept Videos

Test Cross01:39

Test Cross

Alleles are different forms of the same gene. Humans and other diploid organisms inherit two alleles of every gene, one from each parent.
Trihybrid Crosses02:27

Trihybrid Crosses

Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...
Dihybrid Crosses01:18

Dihybrid Crosses

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Monohybrid Crosses01:20

Monohybrid Crosses

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Related Experiment Video

Updated: May 23, 2026

A Method for Characterizing Embryogenesis in Arabidopsis
10:24

A Method for Characterizing Embryogenesis in Arabidopsis

Published on: August 4, 2017

Setting up Arabidopsis crosses.

Detlef Weigel, Jane Glazebrook

    CSH Protocols
    |April 10, 2012
    PubMed
    Summary
    This summary is machine-generated.

    Generating cross-progeny in Arabidopsis requires manual intervention due to natural self-pollination. This protocol details methods for creating and collecting seeds from Arabidopsis crosses for mutant phenotype analysis.

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    Area of Science:

    • Plant Biology
    • Genetics
    • Developmental Biology

    Background:

    • Arabidopsis thaliana is a model organism in plant science.
    • Natural self-pollination in Arabidopsis complicates the generation of cross-progeny.
    • Studying inherited traits often requires analyzing offspring from controlled crosses.

    Purpose of the Study:

    • To provide a detailed protocol for generating and collecting seeds from Arabidopsis crosses.
    • To facilitate the study of mutant phenotypes through controlled genetic crosses.
    • To enable researchers to overcome the challenges of natural self-pollination in Arabidopsis.

    Main Methods:

    • Detailed steps for emasculation of parent flowers.
    • Techniques for artificial pollination between selected Arabidopsis parent plants.
    • Procedures for bagging and collecting developing siliques (seed pods).

    Main Results:

    • Successful generation of viable seeds from controlled Arabidopsis crosses.
    • Demonstration of a reliable method for obtaining cross-progeny.
    • Facilitation of genetic analysis of mutant phenotypes in Arabidopsis.

    Conclusions:

    • This protocol provides a robust method for Arabidopsis cross-pollination.
    • It enables the study of inheritance patterns and gene function.
    • Essential for researchers investigating Arabidopsis genetics and breeding.