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Updated: May 23, 2026
09:00
Detection of Protein Ubiquitination
Published on: August 19, 2009
Detection of ubiquitylated proteins in mammalian cells
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
CSH Protocols
|April 10, 2012
Abstract:
This assay is performed to detect ubiquitylated proteins in transfected mammalian cells. Twenty-four to 40 hours after transfection, cells are harvested, total ubiquitylated proteins are recovered by nickel-affinity chromatography, and specific proteins are detected by Western blotting.
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02:58Regulated Protein Degradation
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
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02:04Covalently Linked Protein Regulators
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
These groups modify specific amino acids in a protein.