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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
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Next-generation sequencing technologies for environmental DNA research.

Shadi Shokralla1, Jennifer L Spall, Joel F Gibson

  • 1Biodiversity Institute of Ontario, Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada. sshokral@uoguelph.ca

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Next-generation sequencing advances have transformed environmental DNA analysis, enabling robust ecological research. This review covers the benefits and drawbacks of current sequencing technologies for environmental DNA applications.

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Area of Science:

  • Biological Sciences
  • Environmental Science
  • Ecology

Background:

  • Next-generation sequencing (NGS) technologies have profoundly impacted biological sciences since 2005.
  • Environmental DNA (eDNA) analysis, utilizing DNA barcodes, is a key application of NGS in ecological research.
  • NGS provides massive sequencing data, improving sample diversity representation cost-effectively.

Purpose of the Study:

  • To review the advantages and limitations of current NGS technologies.
  • To assess the application of NGS in environmental DNA analysis.
  • To highlight the potential of new technologies in accelerating ecological research.

Main Methods:

  • Review of current literature on next-generation sequencing technologies.
  • Analysis of the application of sequencing data in environmental DNA studies.
  • Evaluation of sequencing platforms' read length and throughput improvements.

Main Results:

  • NGS technologies offer significant advantages for eDNA analysis, including increased data volume and improved cost-efficiency.
  • Advancements in read length and throughput enhance the representation of sample diversity.
  • Rapid technological development promises accelerated progress in environmental DNA research.

Conclusions:

  • Current NGS technologies provide powerful tools for environmental DNA analysis, despite existing limitations.
  • Ongoing technological advancements are poised to expand the scope and robustness of eDNA research.
  • The review underscores the critical role of NGS in advancing ecological and environmental monitoring.