Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genomic Diversity of Aurochs From a Mediterranean Ice-Age Refugium.

Molecular ecology·2026
Same author

Lethal plague outbreaks in Lake Baikal hunter-gatherers 5,500 years ago.

Nature·2026
Same author

Population discontinuity in the Paris Basin linked to evidence of the Neolithic decline.

Nature ecology & evolution·2026
Same author

Setting higher standards for reports of microbial species in human cancers.

Nature cancer·2026
Same author

The Contributions of Microbial Interactions to Abrupt Ecosystem Changes during the Late Quaternary.

The American naturalist·2025
Same author

Postdomestication selection of MKK3 shaped seed dormancy and end-use traits in barley.

Science (New York, N.Y.)·2025
Same journal

Differential Immune Responses Correlate With Chytridiomycosis Severity in Italian Crested Newts.

Molecular ecology·2026
Same journal

Demography and Environment Shapes Genetic Variation: Spatiotemporal Genetic Dynamics in Cyclic Voles at Low Latitudes.

Molecular ecology·2026
Same journal

Gut Microbiome-Metabolome Reconfiguration Associates With Phenotypic Plasticity of Daphnia Under Predation Risk.

Molecular ecology·2026
Same journal

Population Genomics Highlight the Vulnerability of Coral-Dwelling Gobies to Ecological Losses due to Climatic Disturbances.

Molecular ecology·2026
Same journal

Ancient Divergences of the Maritime Alpine Tree Larix lyallii (Pinaceae) Contrasts With Patterns in Other Pacific Northwest Coastal Disjuncts.

Molecular ecology·2026
Same journal

Ontogenetic Sequence of Differential Gene Expression in Predator-Induced Daphnia pulex.

Molecular ecology·2026
See all related articles

Related Experiment Video

Updated: May 23, 2026

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles
10:23

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles

Published on: July 11, 2025

Towards next-generation biodiversity assessment using DNA metabarcoding.

Pierre Taberlet1, Eric Coissac, François Pompanon

  • 1Laboratoire d'Ecologie Alpine, CNRS UMR 5553, Université Joseph Fourier, Grenoble, France. pierre.taberlet@ujf-grenoble.fr

Molecular Ecology
|April 11, 2012
PubMed
Summary
This summary is machine-generated.

DNA metabarcoding automates species identification from environmental samples. Future advancements promise to enhance biodiversity research by removing PCR dependency and improving reference libraries.

More Related Videos

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
08:54

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Published on: November 5, 2020

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

Related Experiment Videos

Last Updated: May 23, 2026

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles
10:23

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles

Published on: July 11, 2025

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
08:54

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Published on: November 5, 2020

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

Area of Science:

  • Ecology
  • Genomics
  • Bioinformatics

Background:

  • Species identification is crucial for ecological studies.
  • DNA metabarcoding offers automated, high-throughput species identification from bulk or environmental samples.
  • Current methods are applicable to both modern and ancient samples.

Purpose of the Study:

  • To highlight the emergence and potential of DNA metabarcoding in ecological research.
  • To identify current limitations and future directions for DNA metabarcoding technology.
  • To emphasize the role of next-generation sequencing in advancing taxon identification.

Main Methods:

  • DNA metabarcoding utilizes DNA sequencing to identify multiple species simultaneously.
  • The technique analyzes DNA from environmental samples like soil, water, or feces.
  • Next-generation sequencing platforms facilitate high-throughput data acquisition.

Main Results:

  • DNA metabarcoding is limited by PCR dependence and the need for extensive taxonomic reference libraries.
  • Advancements in DNA sequencing are expected to eliminate the need for DNA amplification.
  • Development of comprehensive reference libraries using curated DNA extracts is ongoing.

Conclusions:

  • DNA metabarcoding is a powerful tool with significant potential for biodiversity research.
  • Future developments will likely streamline the process and expand its applications.
  • Enhanced reference libraries and reduced reliance on PCR will boost data acquisition in ecology.