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Related Experiment Video

Updated: May 23, 2026

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

Published on: June 27, 2022

Reaction based fluorescent probes for hydrogen sulfide.

Chunrong Liu¹, Bo Peng, Sheng Li

¹Department of Chemistry, Washington State University, Pullman, Washington 99164, USA.

Organic Letters

|April 11, 2012

Summary

A new fluorescence turn-on strategy was developed for detecting hydrogen sulfide (H(2)S). This method is highly selective for H(2)S over other biological thiols, offering sensitive detection.

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Area of Science:

Biochemistry
Analytical Chemistry
Chemical Biology

Background:

Hydrogen sulfide (H(2)S) is a crucial signaling molecule in biological systems.
Accurate detection of H(2)S is essential for understanding its physiological roles.
Existing detection methods may lack specificity or sensitivity.

Purpose of the Study:

To develop a novel fluorescence turn-on probe for selective H(2)S detection.
To investigate a reaction mechanism for H(2)S-specific sensing.
To evaluate the probe's performance in biological contexts.

Main Methods:

Design and synthesis of a novel fluorescent probe.
Utilizing a H(2)S-specific Michael addition-cyclization reaction mechanism.
Testing probe selectivity against common biological thiols like cysteine and glutathione.
Assessing probe sensitivity and fluorescence response.

Main Results:

A reaction-based fluorescence turn-on strategy for H(2)S was successfully developed.
The strategy employed a specific Michael addition-cyclization sequence, exclusive to H(2)S.
The probe demonstrated high selectivity, with no significant reaction observed for cysteine or glutathione.
The developed probes exhibited good sensitivity and selectivity for H(2)S detection.

Conclusions:

The developed strategy provides a selective and sensitive method for H(2)S detection.
This approach minimizes interference from other biological thiols.
The findings offer a valuable tool for H(2)S research in chemical biology and biochemistry.