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Related Experiment Video

Updated: May 23, 2026

Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization

Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization

Published on: June 4, 2020

Effect of venipuncture quality on thromboelastography.

Brandi L Garcia-Pereira¹, Michael A Scott, Amy M Koenigshof

¹Departments of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA. Brandi.Garcia@eac-az.com

Journal of Veterinary Emergency and Critical Care (San Antonio, Tex. : 2001)

|April 11, 2012

Summary

Venipuncture quality minimally impacts thromboelastography (TEG) results, with suboptimal collection affecting clot initiation time. Discarding an initial sample tube mitigates these effects in dogs.

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Area of Science:

Veterinary Medicine
Hematology
Diagnostic Testing

Background:

Thromboelastography (TEG) is a valuable tool for assessing hemostasis.
Venipuncture quality can influence laboratory test results.
The impact of venipuncture technique on TEG in dogs is not well-established.

Purpose of the Study:

To evaluate the effect of venipuncture quality on TEG parameters in dogs.
To determine if using a discard tube mitigates potential effects of traumatic venipuncture on TEG results.

Main Methods:

Prospective, observational study involving 15 adult dogs at a veterinary teaching hospital.
Two venipuncture techniques were used: clean venipuncture (CV) and suboptimal venipuncture (SOV).
Two consecutive samples were collected per technique (CV1, CV2, SOV1, SOV2) and analyzed using TEG.

Main Results:

Suboptimal venipuncture (SOV1) significantly shortened the R time (clot initiation) compared to clean venipuncture (CV1).
No significant differences were observed in R time between the second samples (CV2, SOV2) or other TEG parameters (K, α, MA).
The effect of venipuncture trauma on TEG was mild.

Conclusions:

Mild venipuncture trauma has a limited impact on overall TEG results in dogs.
Poorer venipuncture quality can lead to a faster initiation of clot formation, as indicated by a shorter R time.
Collecting a second sample after discarding the initial one effectively mitigates the impact of venipuncture trauma on TEG.