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Updated: May 23, 2026
08:33
Use of Capillary Aerosol Generator in Continuous Production of Controlled Aerosol for Non-Clinical Studies
Published on: April 12, 2022
Charged aerosol detection in pharmaceutical analysis
Stefan Almeling1, David Ilko, Ulrike Holzgrabe
1European Directorate for the Quality of Medicines and Health Care, Strasbourg, France.
Journal of Pharmaceutical and Biomedical Analysis
|April 11, 2012
Summary
Corona-Charged Aerosol Detector (CAD) offers a powerful alternative to UV/vis spectrophotometry for High-Performance Liquid Chromatography (HPLC) detection. This method excels with analytes lacking chromophores, broadening HPLC
Area of Science:
- Analytical Chemistry
- Chromatography
- Spectrophotometry
Background:
- UV/vis spectrophotometry is the standard HPLC detection method due to its broad applicability, sensitivity, linearity, and gradient elution compatibility.
- UV/vis detection faces limitations with analytes lacking chromophores or exhibiting variable UV responses.
- Evaporation/aerosol-based detectors have been developed to overcome these limitations in HPLC.
Purpose of the Study:
- To provide an overview of the Corona-Charged Aerosol Detector (CAD) as an advanced HPLC detection technique.
- To highlight the key performance characteristics of HPLC-CAD.
- To showcase the diverse applications of HPLC-CAD in pharmaceutical analysis.
Main Methods:
- Review of existing literature on Corona-Charged Aerosol Detector (CAD) technology.
- Analysis of performance characteristics, including sensitivity, linearity, and applicability to gradient elution.
- Exploration of various applications within pharmaceutical analysis.
Main Results:
- The Corona-Charged Aerosol Detector (CAD) demonstrates significant potential as a versatile HPLC detector.
- CAD offers a viable solution for detecting analytes that are poorly detected by traditional UV/vis methods.
- Published research indicates broad applicability of HPLC-CAD across different analytical fields, particularly in pharmaceuticals.
Conclusions:
- The Corona-Charged Aerosol Detector (CAD) is a powerful and versatile detector for High-Performance Liquid Chromatography (HPLC).
- HPLC-CAD effectively addresses the limitations of UV/vis detection, especially for analytes with poor chromophoric properties.
- The technology shows significant promise for advancing pharmaceutical analysis.
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