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Updated: May 23, 2026

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection
Published on: February 24, 2026
Triple-color super-resolution imaging of live cells: resolving submicroscopic receptor organization in the plasma
Stephan Wilmes1, Markus Staufenbiel, Domenik Lisse
1Division of Biophysics, Department of Biology, University of Osnabrück, Germany.
Abstract:
In living color: efficient intracellular covalent labeling of proteins with a photoswitchable dye using the HaloTag for dSTORM super-resolution imaging in live cells is described. The dynamics of cellular nanostructures at the plasma membrane were monitored with a time resolution of a few seconds. In combination with dual-color FPALM imaging, submicroscopic receptor organization within the context of the membrane skeleton was resolved.
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