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Micropatterning and Assembly of 3D Microvessels
13:05

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Published on: September 9, 2016

Plasma expanders stabilize human microvessels in microfluidic scaffolds.

Alexander D Leung1, Keith H K Wong, Joe Tien

  • 1Department of Biomedical Engineering, Boston University, 44 Cummington Street, Boston, Massachusetts 02215, USA.

Journal of Biomedical Materials Research. Part A
|April 11, 2012
PubMed
Summary
This summary is machine-generated.

Plasma expanders like dextran and hydroxyethyl starch (HES) stabilize engineered microvessels in scaffolds. These solutions prevent vascular collapse and leakage, maintaining vessel patency for at least two weeks.

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Area of Science:

  • Biomaterials Science
  • Vascular Biology
  • Tissue Engineering

Background:

  • Plasma expanders (dextran, HES) are crucial for maintaining vascular volume and organ preservation.
  • Engineered microvessels in scaffolds are vital for studying vascular biology and for regenerative medicine.

Purpose of the Study:

  • To investigate the stabilizing effects of plasma expanders on engineered microvessels within collagen and fibrin scaffolds.
  • To determine the impact of dextran and HES on microvessel integrity, perfusion, and viability in vitro.

Main Methods:

  • Engineered microvessels were cultured within type I collagen and fibrin scaffolds.
  • Vessels were perfused with standard growth media or media supplemented with dextran or HES.
  • Microvessel integrity, leakage, adhesion, VE-cadherin localization, and patency were assessed over time.

Main Results:

  • Standard media resulted in severe vascular leakage, collapse, and perfusion failure.
  • 3% dextran or 5% HES significantly reduced leaks, maintained scaffold adhesion, and ensured vessel viability and patency for at least 2 weeks.
  • Plasma expanders enhanced VE-cadherin localization at vessel junctions, correlating with stabilization.

Conclusions:

  • Plasma expanders (dextran, HES) physically stabilize engineered microvessels in biomaterial scaffolds.
  • These expanders improve microvessel integrity by enhancing VE-cadherin localization, thereby limiting vascular leakiness.
  • The findings support the use of plasma expanders in tissue engineering applications involving microvascular networks.