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Identification of a selective, activity-based probe for glyceraldehyde 3-phosphate dehydrogenases

Farnusch Kaschani1, Jérôme Clerc, Daniel Krahn

  • 1Zentrum für Medizinische Biotechnologie, Fakultät für Biologie, Universität Duisburg-Essen, Universitätsstrasse 2, 45117 Essen, Germany.

Angewandte Chemie (International Ed. in English)
|April 11, 2012
PubMed
Abstract

No abstract available in PubMed .

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...