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Updated: May 23, 2026

Early Detection of Cyanobacterial Blooms and Associated Cyanotoxins using Fast Detection Strategy
Published on: February 25, 2021
Development of on-line high performance liquid chromatography (HPLC)-biochemical detection methods as tools in the
Christiaan J Malherbe1, Dalene De Beer1, Elizabeth Joubert1,2
1Post-Harvest and Wine Technology Division, Agricultural Research Council, Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch, 7599, South Africa.
Abstract:
Biochemical detection (BCD) methods are commonly used to screen plant extracts for specific biological activities in batch assays. Traditionally, bioactives in the most active extracts were identified through time-consuming bio-assay guided fractionation until single active compounds could be isolated. Not only are isolation procedures often tedious, but they could also lead to artifact formation. On-line coupling of BCD assays to high performance liquid chromatography (HPLC) is gaining ground as a high resolution screening technique to overcome problems associated with pre-isolation by measuring the effects of compounds post-column directly after separation. To date, several on-line HPLC-BCD assays, applied to whole plant extracts and mixtures, have been published. In this review the focus will fall on enzyme-based, receptor-based and antioxidant assays.
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