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Updated: May 23, 2026

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments
Published on: February 4, 2021
Predicting protein crystallizability and nucleation
Nuria Sánchez-Puig1, Claude Sauter, Bernard Lorber
1Instituto de Quimica, Universidad Nacional Autónoma de México, Circuito Exterior, C.U. México, DF 04510 México.
Abstract:
The outcome of protein crystallization attempts is often uncertain due to inherent features of the protein or to the crystallization process that are not fully under control of the experimentalist. The aim of this contribution is to propose user-friendly tools that can increase the success rate of a protein crytallization project. Different bioinformatic approaches to predict the crystallization feasibility (before any crystallization attempts are undertaken) are discussed and a novel approach to assess the nucleation process of a given protein is proposed. Practical examples illustrate these two points.
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