Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Crystal Growth: Principles of Crystallization01:25

Crystal Growth: Principles of Crystallization

Crystallization is a phase transformation process in which crystals are precipitated from a supersaturated solution or formed from other sources. During crystallization, atoms or molecules arrange themselves into a well-defined, rigid crystal lattice to minimize energy.
Initiating crystallization involves manipulating the concentration of the solute and the temperature of the solution. Since crystal growth occurs when the ratio of concentration and solubility of the solute in the solvent – the...
Recrystallization: Solid–Solution Equilibria01:10

Recrystallization: Solid–Solution Equilibria

Recrystallization is a purification technique used to separate impurities from solid compounds. In this technique, no chemical reactions occur. Instead, it exploits physical properties only, specifically, the solubility differences between the desired compound and impurities, either at a single temperature or at different temperatures, and under other selected conditions. The solid-solution equilibrium (solubility equilibrium) of each component in the solution represents a binary phase...
Polymer Classification: Crystallinity01:21

Polymer Classification: Crystallinity

Unlike ionic or small covalent molecules, polymers do not form crystalline solids due to the diffusion limitations of their long-chain structures. However, polymers contain microscopic crystalline domains separated by amorphous domains.
Crystalline domains are the regions where polymer chains are aligned in an orderly manner and held together in proximity by intermolecular forces. For example, chains in the crystalline domains of polyethylene and nylon are bound together by van der Waals...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Chemical Approach to the Synthesis of an Antitumoral Pt-Based Drug Molecule Using a Protein-Ligand Interaction as a Carrier System.

ACS omega·2026
Same author

Hydroxyl radical footprinting modification reveals an intradomain communication pathway in EFL1 disrupted by a Shwachman-Diamond syndrome-associated mutation.

Protein science : a publication of the Protein Society·2026
Same author

Alternative Strategies to Promote Biomolecule Nucleation.

Advances in biochemical engineering/biotechnology·2026
Same author

Integrative Structural Characterization of <i>Candida glabrata</i> Phosphoglycerate Kinase by Small-Angle X‑ray Scattering and AlphaFold: Implications for Therapeutic Targeting in Candidiasis.

ACS omega·2026
Same author

Correction: Salinas-Giegé et al. tRNA Biology in Mitochondria. <i>Int. J. Mol. Sci.</i> 2015, <i>16</i>, 4518-4559.

International journal of molecular sciences·2026
Same author

Structural Implications of Missense Point Mutations in Shwachman-Bodian-Diamond Syndrome Protein (SBDS): A Combined SAXS/MD Investigation.

ACS omega·2025
Same journal

Corrigendum to: Reviewing the Context of Molecular Modeling to Enhance the Application of Machine Learning Technologies for Safer Bioinformatics.

Protein and peptide letters·2026
Same journal

Corrigendum to: A Preliminary Study on the Antibacterial Activity of the Secretion of the Levantine Water Frog, <i>Pelophylax bedriagae</i> (Camerano, 1882) (Anura:Ranidae).

Protein and peptide letters·2026
Same journal

Potent Antioxidant and Antibacterial Activities of ≤3 kDa Hydrolyzed Sarcoplasmic Proteins from IPB-D1 Chicken.

Protein and peptide letters·2026
Same journal

Hybrid 3D Bioprinted Scaffolds for the Delivery of Peptide Therapeutics.

Protein and peptide letters·2026
Same journal

Targeting α-Synuclein: Current Strategies and Emerging Therapies for Synucleinopathies.

Protein and peptide letters·2026
Same journal

Biocompatible Excipients from Microalgae: Advancing Protein and Peptide Therapeutics through Sustainable Formulation Strategies.

Protein and peptide letters·2026
See all related articles

Related Experiment Video

Updated: May 23, 2026

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments
09:52

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments

Published on: February 4, 2021

Predicting protein crystallizability and nucleation.

Nuria Sánchez-Puig1, Claude Sauter, Bernard Lorber

  • 1Instituto de Quimica, Universidad Nacional Autónoma de México, Circuito Exterior, C.U. México, DF 04510 México.

Protein and Peptide Letters
|April 12, 2012
PubMed
Summary
This summary is machine-generated.

Predicting protein crystallization success is challenging. This study introduces user-friendly bioinformatics tools to improve crystallization feasibility prediction and assess protein nucleation, aiming to increase success rates in structural biology projects.

More Related Videos

Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
09:15

Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering

Published on: August 14, 2018

Protein Crystallization for X-ray Crystallography
09:27

Protein Crystallization for X-ray Crystallography

Published on: January 16, 2011

Related Experiment Videos

Last Updated: May 23, 2026

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments
09:52

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments

Published on: February 4, 2021

Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
09:15

Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering

Published on: August 14, 2018

Protein Crystallization for X-ray Crystallography
09:27

Protein Crystallization for X-ray Crystallography

Published on: January 16, 2011

Area of Science:

  • Structural Biology
  • Biophysics
  • Computational Biology

Background:

  • Protein crystallization is crucial for structural determination but often faces unpredictable outcomes.
  • Experimental success is limited by inherent protein properties and process variability.
  • Developing predictive tools can significantly enhance the efficiency of protein structure determination.

Purpose of the Study:

  • To propose user-friendly bioinformatics tools to improve the success rate of protein crystallization projects.
  • To provide methods for predicting crystallization feasibility prior to experimental work.
  • To introduce a novel approach for assessing the nucleation phase of protein crystallization.

Main Methods:

  • Discussion of various bioinformatic approaches for predicting protein crystallization feasibility.
  • Proposal of a novel computational method to evaluate the nucleation process.
  • Illustration of proposed methods with practical examples.

Main Results:

  • Bioinformatic tools can predict crystallization feasibility before experiments.
  • A novel method aids in assessing the critical nucleation step.
  • Practical examples demonstrate the utility of the proposed approaches.

Conclusions:

  • User-friendly bioinformatics tools can enhance protein crystallization success rates.
  • Predictive modeling of crystallization feasibility and nucleation is valuable for structural biology.
  • These tools offer practical solutions for experimentalists facing crystallization challenges.