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Related Concept Videos

X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...
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Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Atomic Absorption Spectroscopy: Radiation and Light Sources01:13

Atomic Absorption Spectroscopy: Radiation and Light Sources

Atomic absorption spectroscopy (AAS) relies on the Beer-Lambert law, which requires that the radiation source emits a narrow range of wavelengths to match the absorption characteristics of the analyte atom. The primary criteria for choosing an appropriate radiation source in AAS is to provide a precise and intense emission at specific wavelengths that will allow accurate detection of the analyte.
Two common narrow-range 'line' sources used in AAS are hollow-cathode lamps (HCLs) and...

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Updated: May 23, 2026

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

Synchrotron radiation in life sciences.

Vivian Stojanoff1, Paul Northrup, Ruth Pietri

  • 1Brookhaven National Laboratory, National Synchrotron Light Source, Building 725D, Upton NY 11973, USA. stojanoff@bnl.gov

Protein and Peptide Letters
|April 12, 2012
PubMed
Summary
This summary is machine-generated.

Synchrotron Radiation (SR) offers diverse methods for life science research. This study introduces SR diffraction, spectroscopy, and imaging techniques to complement laboratory work for biological molecule analysis.

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Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers
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Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
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Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

Published on: June 19, 2018

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Last Updated: May 23, 2026

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08:46

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Published on: April 13, 2016

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Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers

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Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
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Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

Published on: June 19, 2018

Area of Science:

  • Life Sciences
  • Biophysics
  • Structural Biology

Background:

  • Synchrotron Radiation (SR) provides advanced experimental capabilities.
  • Traditional laboratory methods have limitations in analyzing complex biological systems.
  • SR offers a unique environment for detailed molecular investigation.

Purpose of the Study:

  • To introduce key Synchrotron Radiation (SR) methods relevant to life sciences.
  • To highlight SR's complementary role to home laboratory techniques.
  • To showcase SR's utility in atomic structure determination and functional characterization.

Main Methods:

  • Synchrotron Radiation (SR) diffraction for atomic structure.
  • SR spectroscopy for chemical compound characterization.
  • SR imaging for macromolecular analysis.

Main Results:

  • Demonstration of SR diffraction, spectroscopy, and imaging techniques.
  • Application of these methods to biological macromolecules.
  • Insights into the properties and function of biologically relevant compounds.

Conclusions:

  • SR offers a powerful 'playground' for life science research.
  • SR techniques significantly enhance the study of biological molecules.
  • These methods are crucial for advancing our understanding of life's mysteries.