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Updated: Dec 29, 2025

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Published on: July 17, 2021

Genetic modification of baculovirus expression vectors.

Shu-fen Li¹, Hua-lin Wang, Zhi-hong Hu

¹State Key Laboratory of Virology and Joint-Lab of Invertebrate Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.

Virologica Sinica

|April 12, 2012

Summary

Baculoviral vectors are versatile tools for protein expression in insect and mammalian cells. Genetic modifications enhance their utility for high-level protein production and targeted gene delivery, making them increasingly accessible.

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Last Updated: Dec 29, 2025

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

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Area of Science:

Biotechnology
Molecular Biology
Virology

Background:

Baculoviruses are widely used as protein expression vectors.
Genetic engineering has expanded their capabilities for heterologous protein production.

Purpose of the Study:

To review the development and current state of baculovirus expression systems.
To highlight genetic modifications enhancing protein expression and gene delivery.

Main Methods:

Review of literature on baculovirus vector modifications.
Analysis of strategies for improving protein expression and cell targeting.

Main Results:

Baculoviral vectors have been modified using various promoters, signal peptides, and gene deletions/replacements.
Techniques like pseudotyping and surface display enhance mammalian cell targeting.
Polycistronic cassettes enable production of protein complexes.

Conclusions:

Baculovirus expression systems offer significant advantages over other vectors.
Ongoing advancements promise greater feasibility and accessibility for advanced applications.