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Related Experiment Video

Updated: May 23, 2026

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size
13:46

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size

Published on: October 17, 2016

Does the tissue engineering architecture of poly(3-hydroxybutyrate) scaffold affects cell-material interactions?

Elahe Masaeli1, Mohammad Morshed, Parsa Rasekhian

  • 1Department of Textile Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.

Journal of Biomedical Materials Research. Part A
|April 12, 2012
PubMed
Summary

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Poly(3-hydroxybutyrate) (PHB) scaffolds were fabricated using electrospinning and salt-leaching. Nanofibrous scaffolds showed better cell attachment, while salt-leached scaffolds enhanced Vero cell proliferation, indicating suitability for tissue engineering.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Polymer Chemistry

Background:

  • Three-dimensional scaffolds are crucial for tissue engineering, mimicking the extracellular matrix to support cell growth.
  • Poly(3-hydroxybutyrate) (PHB), a type of polyhydroxyalkanoate (PHA), offers tunable biocompatibility and biodegradability for regenerative medicine.
  • Fabrication methods significantly influence scaffold properties and cellular responses.

Purpose of the Study:

  • To fabricate and compare PHB scaffolds using electrospinning and salt-leaching techniques.
  • To evaluate the physical, mechanical, and biological properties of the fabricated scaffolds.
  • To assess the suitability of different scaffold types for tissue engineering applications.

Main Methods:

  • Poly(3-hydroxybutyrate) (PHB) scaffolds were fabricated via electrospinning and salt-leaching.

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Self-reporting Scaffolds for 3-Dimensional Cell Culture
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Self-reporting Scaffolds for 3-Dimensional Cell Culture

Published on: November 7, 2013

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Last Updated: May 23, 2026

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size
13:46

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size

Published on: October 17, 2016

Self-reporting Scaffolds for 3-Dimensional Cell Culture
14:49

Self-reporting Scaffolds for 3-Dimensional Cell Culture

Published on: November 7, 2013

  • Scaffold properties were analyzed using MTS assay, scanning electron microscopy, tensile strength, contact angle, and porosity measurements.
  • Cellular behavior of monkey epithelial kidney cells (Vero) and mouse mesenchymal stem cells (mMSCs) was investigated.

    • Main Results:

    • Salt-leached scaffolds exhibited higher wettability and permeability but lower mechanical strength compared to nanofibrous scaffolds.
    • Vero cell proliferation was enhanced on salt-leached scaffolds.
    • Both scaffold types supported similar proliferation rates for mouse mesenchymal stem cells (mMSCs).

    Conclusions:

    • Nanofibrous PHB scaffolds demonstrate superior potential for cell attachment and morphology.
    • Salt-leached PHB scaffolds show promise for applications requiring enhanced cell proliferation, particularly for specific cell types like Vero cells.
    • The choice of scaffold fabrication method critically impacts material properties and cellular interactions in tissue engineering.