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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

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Related Experiment Video

Updated: May 23, 2026

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis

Published on: January 27, 2021

Novel microRNAs encoded by duck enteritis virus.

Yongxiu Yao¹, Lorraine P Smith¹, Lawrence Petherbridge¹

¹Viral Oncogenesis Group, Institute for Animal Health, Compton, Berkshire RG20 7NN, UK.

The Journal of General Virology

|April 12, 2012

Summary

Researchers discovered novel duck enteritis virus (DEV)-encoded microRNAs (miRNAs) using deep sequencing. These DEV miRNAs, primarily located in the unique long genome region, offer new insights into viral pathogenesis.

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Published on: February 6, 2017

Area of Science:

Virology
Molecular Biology
Genomics

Background:

Duck enteritis virus (DEV) causes a severe, contagious disease in waterfowl.
Understanding virus-host interactions is crucial for disease management.

Purpose of the Study:

To identify novel microRNAs (miRNAs) encoded by the duck enteritis virus (DEV).
To investigate the genomic location and potential function of these novel DEV-encoded miRNAs.

Main Methods:

Deep sequencing of RNA from DEV-infected chicken embryo fibroblast cultures.
Bioinformatic analysis to identify and map viral miRNAs.
Functional reporter assays to assess miRNA activity.

Main Results:

Several novel DEV-encoded miRNAs were identified.
DEV miRNAs predominantly mapped to the unique long (UL) region of the viral genome.
DEV miR-D18-3p demonstrated functional activity in reporter assays.

Conclusions:

The study expands the known repertoire of virus-encoded miRNAs.
Novel DEV miRNAs, particularly those in the UL region, warrant further investigation for their role in DEV pathogenesis.