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Updated: May 23, 2026
08:31
Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
Published on: May 26, 2013
Complete genome sequence of enterococcal bacteriophage SAP6.
Young-Duck Lee1, Jong-Hyun Park
1Department of Food Science and Biotechnology, College of Engineering, Gachon University, Sungnam-Si, Kyunggi-Do, South Korea.
Journal of Virology
|April 12, 2012
Summary
Researchers identified a novel bacteriophage, SAP6, effective against multidrug-resistant Enterococcus faecalis. This discovery offers a potential new strategy for combating antibiotic-resistant bacterial infections.
Area of Science:
- Microbiology
- Bacteriology
- Genomics
Background:
- Enterococcus faecalis is a significant bacterium with dual roles as a probiotic and opportunistic pathogen.
- Multidrug-resistant strains of E. faecalis pose a growing threat in clinical settings.
- There is a critical need for alternative therapeutic strategies against antibiotic-resistant bacteria.
Purpose of the Study:
- To identify novel therapeutic agents against multidrug-resistant Enterococcus faecalis.
- To characterize a newly isolated enterococcal bacteriophage.
- To determine the complete genome sequence of bacteriophage SAP6.
Main Methods:
- Screening for bacteriophages active against multidrug-resistant E. faecalis.
- Isolation and characterization of virulent enterococcal bacteriophage SAP6.
- Whole-genome sequencing of bacteriophage SAP6.
Main Results:
- A virulent bacteriophage, SAP6, belonging to the Siphoviridae family, was isolated.
- The complete genome sequence of bacteriophage SAP6 was determined for the first time.
- Bacteriophage SAP6 demonstrated potential as a therapeutic agent.
Conclusions:
- Bacteriophage SAP6 represents a promising candidate for combating multidrug-resistant E. faecalis.
- The findings suggest bacteriophage therapy as a viable alternative or adjunct to conventional antibiotics.
- Further research into bacteriophage SAP6 could lead to new treatments for resistant bacterial infections.
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