Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cell Polarization by Rho Proteins

Cell Polarization by Rho Proteins

Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...

Regulation of Nuclear Protein Sorting

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

Negative Regulator Molecules

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.

Small GTPases - Ras and Rho

Small GTPases - Ras and Rho

Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Explainable AI for Equitable Nurse Scheduling: Pragmatic Pre-Post Implementation Study.

JMIR nursing·2026

Same author

Association between pre-diagnostic prevalence of 30 common diseases and the subsequent risk of dementia: a population-based retrospective cohort study in Taiwan.

Frontiers in aging neuroscience·2026

Same author

Enhancing Model Generalizability in Medical Artificial Intelligence: Systematic Comparison of Categorical Encoding and Sampling Techniques for Imbalanced Data.

JMIR medical informatics·2026

Same author

Chinese Herbal Medicine Is Associated With Improved Survival in Patients With Advanced Lung Cancer Receiving Standard Treatment: A Nationwide Population-Based Propensity Score Matching Study.

Integrative cancer therapies·2026

Same author

Dose-response effect of statins on colorectal cancer risk in IBD: a nationwide cohort study.

BMC cancer·2026

Same author

Machine Learning Prediction of Progression to Dialysis in Patients With Polycystic Kidney Disease: Population-Based Retrospective Cohort Study.

JMIR medical informatics·2026

Search research articles

Related Experiment Video

Updated: May 23, 2026

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Published on: March 17, 2014

hnRNP Q regulates Cdc42-mediated neuronal morphogenesis.

Hung-Hsi Chen¹, Hsin-I Yu, Wen-Cheng Chiang

¹Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.

Molecular and Cellular Biology

|April 12, 2012

Summary

Knocking down hnRNP Q enhances neuronal complexity and filopodium formation by regulating mRNA localization. This suggests hnRNP Q1 controls actin dynamics and neuronal morphogenesis.

More Related Videos

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons

A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons

Published on: August 6, 2020

Related Experiment Videos

Last Updated: May 23, 2026

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Published on: March 17, 2014

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons

A Neurite Outgrowth Assay and Neurotoxicity Assessment with Human Neural Progenitor Cell-Derived Neurons

Published on: August 6, 2020

Area of Science:

Neuroscience
Molecular Biology
Cell Biology

Background:

The RNA-binding protein hnRNP Q is involved in neuronal mRNA metabolism.
Neuronal morphogenesis is a complex process influenced by mRNA regulation.

Purpose of the Study:

To investigate the role of hnRNP Q1 in neuronal morphogenesis.
To identify mRNA targets of hnRNP Q1 and elucidate its mechanism of action.

Main Methods:

Cell culture (rat cortical neurons, mouse neuroblastoma cells)
RNA-binding protein knockdown and reexpression
mRNA target identification and binding sequence analysis
Subcellular fractionation and immunofluorescence
Analysis of actin nucleation-promoting complex (Cdc42/N-WASP/Arp2/3) and dominant-negative mutants

Main Results:

hnRNP Q knockdown increased neurite complexity and filopodium formation.
hnRNP Q1 specifically binds to mRNAs encoding components of the Cdc42/N-WASP/Arp2/3 complex.
hnRNP Q knockdown reduced mRNA levels in neurites and altered protein distribution.
Inhibition of Cdc42 or N-WASP counteracted hnRNP Q depletion-induced effects.

Conclusions:

hnRNP Q1 plays a role in neuronal morphogenesis.
hnRNP Q1 may regulate actin dynamics by controlling the localization of specific mRNAs in neurites.
These findings provide insights into the molecular mechanisms governing neuronal development.