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Related Experiment Video

Updated: May 23, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

A cell surfaceome map for immunophenotyping and sorting pluripotent stem cells.

Rebekah L Gundry1, Daniel R Riordon, Yelena Tarasova

  • 1Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA.

Molecular & Cellular Proteomics : MCP
|April 12, 2012
PubMed
Summary
This summary is machine-generated.

Researchers identified 500 cell surface proteins to distinguish high-quality induced pluripotent stem cells (iPSCs) for regenerative medicine. This discovery aids in isolating valuable iPSC lines from mixed cultures.

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Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2

Published on: December 6, 2013

Area of Science:

  • Stem cell biology
  • Proteomics
  • Regenerative medicine

Background:

  • Induced pluripotent stem cells (iPSCs) hold promise for regenerative medicine but exhibit heterogeneity, limiting their applications.
  • Current methods for assessing iPSC quality are insufficient, necessitating new approaches for distinguishing cell lines.

Purpose of the Study:

  • To develop rapid, non-mutagenic methods for identifying and isolating high-quality induced pluripotent stem cells (iPSCs).
  • To expand the knowledge of cell surface proteins and accessible epitopes on pluripotent stem cells.

Main Methods:

  • Utilized a chemoproteomic targeting strategy for de novo discovery of cell surface N-glycoproteins.
  • Identified approximately 500 cell surface proteins across embryonic stem cell and iPSC lines.
  • Applied immunophenotyping, cell sorting, and functional analyses to validate identified markers.

Main Results:

  • Successfully identified 500 cell surface proteins on pluripotent stem cells.
  • Demonstrated the utility of these markers in isolating iPSCs from heterogeneous reprogrammed cell populations.
  • Showcased the ability to isolate functionally distinct stem cell subpopulations using the identified markers.

Conclusions:

  • The newly identified surface marker panels are effective for isolating high-quality iPSCs.
  • This resource facilitates the selection of suitable iPSC lines for disease modeling, drug discovery, and therapeutic applications.
  • Advances in stem cell surface marker discovery pave the way for improved regenerative medicine strategies.