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Related Experiment Video

Updated: May 23, 2026

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Published on: November 22, 2024

Tissue factor expression in the symptomatic carotid plaque.

Hani Abdul-Jabar¹, Abbas Rashid, Amir Sadri

¹Department of Vascular Surgery, The Hillingdon Hospital, Pield Heath Road, Uxbridge, Middlesex UB8 3NN, UK.

Journal of Clinical Medicine Research

|April 12, 2012

Summary

Symptomatic carotid stenosis patients show increased tissue factor in plaques, suggesting a mechanism for plaque instability and stroke risk. This highlights the importance of plaque stability in understanding carotid stenosis progression.

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A Human Ex Vivo Atherosclerotic Plaque Model to Study Lesion Biology

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Last Updated: May 23, 2026

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

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Published on: November 22, 2024

A Human Ex Vivo Atherosclerotic Plaque Model to Study Lesion Biology

A Human Ex Vivo Atherosclerotic Plaque Model to Study Lesion Biology

Published on: May 6, 2014

Area of Science:

Vascular biology and pathology
Neurology and stroke research
Biomarkers in cardiovascular disease

Background:

Carotid stenosis, a narrowing of the carotid arteries, presents differently in symptomatic versus asymptomatic patients.
Differences in disease progression may stem from varying levels of procoagulant protein factors.
Tissue factor is a key protein implicated in blood clot formation.

Purpose of the Study:

To investigate if symptomatic and asymptomatic carotid stenosis patients exhibit differences in tissue factor expression within carotid artery plaques.
To explore the relationship between tissue factor levels and plaque characteristics in patients with severe carotid stenosis.

Main Methods:

Carotid artery plaques were collected from 33 symptomatic and 4 asymptomatic patients with >70% internal carotid artery stenosis.
Plaques were immunostained using a monoclonal antibody targeting human tissue factor.
Stained areas in the plaque cap and core were quantified using specialized computer analysis.

Main Results:

A statistically significant increase in tissue factor staining was observed in plaques from symptomatic patients compared to asymptomatic ones (P = 0.0001).
Within the symptomatic group, recently symptomatic patients showed significantly higher tissue factor levels in the plaque core (P = 0.003).
The study included 37 patients (27 male, mean age 69.3 years, range 53-83 years).

Conclusions:

Unstable carotid artery plaques are associated with significantly elevated tissue factor expression in both the cap and core.
Higher tissue factor in the core of plaques from recently symptomatic patients may contribute to plaque destabilization.
Further research is warranted to fully elucidate the role of tissue factor in carotid plaque instability and stroke pathogenesis.