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Cell Co-culture Patterning Using Aqueous Two-phase Systems

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Published on: March 26, 2013

Aqueous two-phase systems for protein separation: phase separation and applications.

Juan A Asenjo¹, Barbara A Andrews

¹Centre for Biochemical Engineering and Biotechnology, Department of Chemical Engineering and Biotechnology, Institute for Cell Dynamics and Biotechnology: A Centre for Systems Biology, University of Chile, Santiago, Chile.

Journal of Chromatography. A

|April 13, 2012

Summary

Aqueous two-phase systems (ATPS) offer efficient protein purification. This review details phase separation, continuous processing, and mathematical modeling for large-scale applications, ensuring high purity and yield in bioprocessing.

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Area of Science:

Biochemical Engineering
Separation Science
Process Chemistry

Background:

Aqueous two-phase systems (ATPS) are effective for protein separation and purification.
Protein partitioning in ATPS depends on protein surface properties and system composition.
Industrial applications demonstrate high purity and yield using ATPS.

Purpose of the Study:

To review key elements for implementing ATPS in bioprocessing.
To analyze phase separation and continuous processing aspects of ATPS.
To highlight the importance of mathematical modeling for scale-up.

Main Methods:

Analysis of phase separation behavior in polymer-salt ATPS.
Investigation of phase separation kinetics based on physical properties (viscosity, density, interfacial tension).
Development of mathematical models for continuous, steady-state ATPS operation.
Simulations to evaluate the effect of phase ratio on purification.

Main Results:

Phase separation in PEG-salt ATPS depends on the continuous phase.
Dispersion height profiles are independent of separator dimensions, aiding large-scale design.
Settling rates correlate with physical properties, enabling equipment design.
Mathematical models effectively simulate continuous ATPS operation and purification.

Conclusions:

Continuous bottom-phase operation ensures rapid separation.
ATPS are a proven technology for industrial-scale protein purification with high purity and yield.
ATPS are applicable to a wide range of biomolecules, including recombinant proteins and antibodies.