Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Diversity of Chlamydiaceae among native and introduced vertebrate populations from sub-Antarctic islands.

Veterinary microbiology·2026

Same author

Genomic and morphological analysis of human clinical Burkholderia pseudomallei isolates from New Caledonia (2021-2022).

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases·2026

Same author

Genome sequences of 15 <i>Escherichia coli</i> O77g strains related to an emerging STEC lineage.

Microbiology resource announcements·2026

Same author

Analysis of the genetic and phylogenetic context of <i>Escherichia coli</i> O77g:H18 associated with clustered cases of HUS in France in 2025.

Applied and environmental microbiology·2026

Same author

Occurrence of viral and bacterial pathogens in South American camelids in representative German flocks.

Frontiers in veterinary science·2026

Same author

Emergence of distinct Burkholderia pseudomallei lineages in Brazil: Phenotypic, genotypic, and phylogenetic agreement.

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases·2026

Search research articles

Related Experiment Video

Updated: May 23, 2026

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Published on: June 17, 2019

Multilocus variable-number tandem-repeat analysis scheme for chlamydia felis genotyping: comparison with multilocus

Karine Laroucau¹, Antonietta Di Francesco, Fabien Vorimore

¹Bacterial Zoonoses Unit, French Agency for Food, Environmental & Occupational Health Safety (ANSES), Maisons-Alfort, France. karine.laroucau@anses.fr

Journal of Clinical Microbiology

|April 13, 2012

Summary

A new multilocus variable-number tandem-repeat analysis (MLVA) system effectively differentiates Chlamydia felis isolates. This high-resolution test is crucial for tracking feline Chlamydia infections in global veterinary epidemiology.

More Related Videos

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)

Published on: July 15, 2011

Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow

Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow

Published on: August 15, 2025

Related Experiment Videos

Last Updated: May 23, 2026

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Published on: June 17, 2019

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)

Published on: July 15, 2011

Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow

Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow

Published on: August 15, 2025

Area of Science:

Veterinary Microbiology
Molecular Epidemiology
Ocular Pathogens

Background:

Chlamydia felis is a significant cause of feline ocular disease globally.
Accurate strain differentiation is essential for understanding disease transmission and control.

Purpose of the Study:

To develop and validate a novel multilocus variable-number tandem-repeat analysis (MLVA) system for Chlamydia felis.
To assess the system's utility in differentiating C. felis isolates for molecular epidemiology.

Main Methods:

Development of an MLVA system targeting tandem repeats across the C. felis genome.
Testing the MLVA system on 17 C. felis isolates and 122 clinical samples.
Comparison with a multilocus sequence typing (MLST) scheme on select samples.

Main Results:

The developed MLVA system identified 25 distinct C. felis MLVA patterns.
High-resolution differentiation of isolates from various geographic origins was achieved.
MLVA results showed good concordance with the MLST scheme, with rare sequence differences observed.

Conclusions:

The novel MLVA system offers a highly sensitive and high-resolution method for C. felis strain typing.
This tool is well-suited for molecular epidemiological studies of feline Chlamydia.
The system aids in differentiating C. felis isolates, contributing to disease control strategies.