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Related Experiment Video

Updated: May 23, 2026

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

Published on: November 12, 2015

Replication-uncoupled histone deposition during adenovirus DNA replication.

Tetsuro Komatsu¹, Kyosuke Nagata

¹Department of Infection Biology, Faculty of Medicine and Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.

Journal of Virology

|April 13, 2012

Summary

Viral DNA in infected cells associates with histones during both early and late infection stages. Histone variant H3.3 is selectively bound, suggesting replication-uncoupled histone deposition.

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Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

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Published on: August 31, 2017

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Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

Published on: November 12, 2015

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

Area of Science:

Molecular Biology
Virology
Epigenetics

Background:

Chromatin structure of adenovirus DNA is crucial for genome functions in infected cells.
Incoming viral DNA associates with viral protein VII and histones in early infection phases.

Purpose of the Study:

To investigate histone association with newly synthesized viral DNA in late infection phases.
To explore the role of histone chaperones and DNA binding proteins in viral chromatin formation.

Main Methods:

Chromatin immunoprecipitation assays with epitope-tagged histone H3.
Microscopic analyses of histone and transcription factor localization.
Knockdown of CAF-1 (chromatin assembly factor-1) and observation of its effect on histone binding.

Main Results:

Newly synthesized adenovirus DNA associates with histones in late infection.
Histone variant H3.3 is selectively bound to both incoming and newly synthesized viral DNA.
CAF-1 knockdown does not alter histone H3 levels on viral chromatin, despite CAF-1 accumulation at replication foci.
Histones, but not USF1, are excluded from viral DNA replication foci, mediated by DNA binding protein (DBP) oligomerization.

Conclusions:

Histone deposition onto newly synthesized viral DNA appears uncoupled from viral DNA replication.
DBP oligomerization may play a role in this replication-uncoupled histone deposition process.