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Related Experiment Video

Updated: May 23, 2026

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Published on: November 1, 2017

Efficient commitment to functional CD34+ progenitor cells from human bone marrow mesenchymal stem-cell-derived

Yulin Xu¹, Lizhen Liu, Lifei Zhang

¹Bone Marrow Transplantation Center, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China.

|April 13, 2012

Summary

Researchers efficiently generated CD34+ progenitor cells from human bone marrow mesenchymal stem cell-induced pluripotent stem cells (hBMMSC-iPSCs). These cells show hematopoietic and endothelial potential, crucial for iPSC therapy and disease modeling.

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Last Updated: May 23, 2026

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

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Published on: November 1, 2017

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

Pan-myeloid Differentiation of Human Cord Blood Derived CD34⁺ Hematopoietic Stem and Progenitor Cells

Published on: August 9, 2019

Area of Science:

Stem Cell Biology
Hematopoiesis
Cell Therapy

Background:

Clinical applications of induced pluripotent stem cells (iPSCs) require efficient generation of specialized cell types without contaminants.
CD34+ progenitor cells possess hematopoietic and endothelial potential, making them valuable for regenerative medicine.

Purpose of the Study:

To efficiently derive CD34+ progenitor cells with both hematopoietic and endothelial potential from human bone marrow mesenchymal stem cell-derived iPSCs (hBMMSC-iPSCs) using defined factors.
To assess the differentiation efficiency and potential of these derived progenitor cells.

Main Methods:

Treatment of hBMMSC-iPSCs with a cocktail of mesodermal, hematopoietic, and endothelial inducers (BMP4, SCF, VEGF) for 5 days.
Further culture with a second cocktail (SCF, Flt3L, VEGF, IL-3) for 7-9 days to promote progenitor cell formation.
Flow cytometry, transcription factor analysis (Brachyury, GATA-2, TAL-1), colony formation assays, and endothelial marker assessment (CD31, VE-CADHERIN).

Main Results:

hBMMSC-iPSCs treated with the initial cocktail showed increased expression of mesodermal transcription factors Brachyury and GATA-2.
Nearly 20% of cells differentiated into CD34+ progenitor cells, a significantly higher yield compared to human skin fibroblast-derived iPSCs (hFib-iPSCs) or spontaneous differentiation.
Induced CD34+ cells exhibited hematopoietic potential (colony formation) and endothelial potential (tube formation, marker expression).

Conclusions:

Defined factor induction enables efficient generation of CD34+ progenitor cells from hBMMSC-iPSCs, retaining both hematopoietic and endothelial potential.
This method offers a pathway for patient-specific cell generation for iPSC therapy.
The derived cells serve as a valuable model for studying hematopoiesis and for drug screening.