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Updated: May 23, 2026
09:20
Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
Published on: March 31, 2016
Changes in gene expression in a porcine preadipocyte cell line during differentiation
T Matsumoto1, I Nakajima, T Eguchi-Ogawa
1Animal Research Division, Institute of Society for Techno-innovation of Agriculture, Forestry and Fisheries, 446-1 Kamiyokoba, Tsukuba, Ibaraki, 305-0854, Japan.
Animal Genetics
|April 14, 2012
Summary
Understanding pig gene expression during fat cell differentiation is key for improving meat quality. This study identifies key genes and their roles in adipocyte differentiation for molecular breeding.
Area of Science:
- Animal Science
- Molecular Biology
- Genetics
Background:
- Adipocyte differentiation is crucial for fat tissue development in pigs, impacting meat quality and productivity.
- Identifying specific pig genes involved offers insights for dietary regulation of fat content and breeding strategies.
Purpose of the Study:
- To investigate time-dependent gene expression changes during porcine adipocyte differentiation.
- To identify genes associated with fat traits and potential targets for molecular breeding.
Main Methods:
- Construction of a DNA oligomer microarray based on pig transcripts.
- Analysis of gene expression in the PSPA porcine preadipocyte cell line during differentiation.
- Selection of genes with at least a fivefold change in expression.
Main Results:
- Gene expression patterns were classified into five groups based on differentiation stage.
- Early differentiation showed up-regulation of genes involved in cell proliferation and transcription.
- 27 significantly altered transcripts were located within quantitative trait loci (QTL) regions for adipose traits.
Conclusions:
- The study provides a valuable resource for identifying genes responsible for fat-related traits in pigs.
- Findings can guide molecular breeding efforts to enhance fat traits and meat quality.
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