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Purification and biochemical characterization of functional complement factor H from human plasma fractions
H Brandstätter1, P Schulz, I Polunic
1Octapharma PPGmbH, Research & Development, Vienna, Austria. hubert.brandstaetter@octapharma.com
Vox Sanguinis
|April 14, 2012
Summary
A new scalable purification process yields pathogen-safe, functional complement factor H (CFH) concentrate from human plasma. This method removes dysfunctional CFH, providing a reliable source for clinical studies.
Area of Science:
- Biochemistry
- Immunology
- Protein Purification
Background:
- Complement factor H (CFH) regulates the alternative complement pathway.
- CFH mutations and polymorphisms are linked to various human diseases.
Purpose of the Study:
- To develop a scalable purification process for human plasma-derived CFH.
- To produce a pathogen-safe and functional CFH concentrate.
Main Methods:
- Purification involved three chromatographic steps starting from plasma intermediates.
- Included solvent detergent treatment and nanofiltration for pathogen safety.
- CFH functionality assessed via hemolysis, decay acceleration, and cofactor activity assays.
Main Results:
- Achieved high purification of full-length, native CFH.
- Successfully removed truncated and dysfunctional CFH species.
- Demonstrated functional CFH concentrate through in vitro assays.
Conclusions:
- Developed a novel, scalable process for CFH concentrate production.
- The concentrate is pathogen-safe and functionally active.
- Suitable for preclinical and clinical studies.
