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Related Experiment Video

Updated: May 23, 2026

Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

Graphene-based high-efficiency surface-enhanced Raman scattering-active platform for sensitive and multiplex DNA

Shijiang He1, Keng-Ku Liu, Shao Su

  • 1Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.

Analytical Chemistry
|April 14, 2012
PubMed
Summary

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We created a novel substrate using gold nanoparticles and graphene for highly sensitive DNA detection. This surface-enhanced Raman scattering (SERS) platform enables simultaneous detection of two DNA targets with excellent specificity.

Area of Science:

  • Nanotechnology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Direct immobilization of DNA on graphene films presents challenges.
  • Surface-enhanced Raman scattering (SERS) offers high sensitivity for molecular detection.
  • Multiplexing detection requires advanced substrate materials.

Purpose of the Study:

  • To develop a novel SERS-active substrate for enhanced DNA detection.
  • To utilize gold nanoparticles and graphene for improved signal amplification.
  • To achieve sensitive and specific multiplexing detection of DNA targets.

Main Methods:

  • Fabrication of a SERS-active substrate by decorating graphene with gold nanoparticles.
  • Assembly of DNA capture probes onto the gold nanoparticle-graphene surface.

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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

Related Experiment Videos

Last Updated: May 23, 2026

Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

  • Utilizing SERS for the detection of DNA targets.

    • Main Results:

    • The novel substrate demonstrated dramatically enhanced Raman signals.
    • Extraordinarily high sensitivity with a detection limit as low as 10 pM was achieved.
    • Simultaneous detection of two different DNA targets using a single light source was successfully demonstrated.

    Conclusions:

    • The gold nanoparticle-decorated CVD-graphene substrate is highly effective for sensitive and specific DNA detection.
    • This SERS platform overcomes limitations of direct DNA immobilization on graphene.
    • The developed substrate shows significant potential for multiplexed biosensing applications.