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Published on: October 10, 2013
Analysis of differentially expressed proteins involved in hand, foot and mouth disease and normal sera
Insights
Researchers identified key proteins in children with hand, foot, and mouth disease (HFMD) serum. This proteomic profile offers insights into disease mechanisms and regulatory networks for HFMD.
Area of Science:
- Proteomics
- Biochemistry
- Pediatric Infectious Diseases
Background:
- Hand, foot, and mouth disease (HFMD) is a common viral illness in children.
- Understanding the molecular changes in HFMD is crucial for developing effective treatments.
- Serum protein profiles can serve as biomarkers for disease diagnosis and severity.
Purpose of the Study:
- To identify differentially expressed proteins in serum from children with mild and severe HFMD compared to healthy controls.
- To analyze the functional associations of these identified proteins.
- To establish a comprehensive proteomic profile of HFMD.
Main Methods:
- Two-dimensional difference gel electrophoresis (2-D DIGE) was used to analyze serum proteins.
- Matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS) was employed for protein identification.
- Bioinformatic analyses, including classification and protein-protein interaction mapping, were performed.
Main Results:
- 30 differentially expressed proteins were identified in mild HFMD serum and 39 in severe HFMD serum.
- 25 proteins were common to both mild and severe HFMD groups, with 14 up-regulated and 11 down-regulated.
- These proteins are associated with functions such as transporter activity and catalytic activity.
Conclusions:
- The study provides a detailed proteomic profile of HFMD in children.
- The identified proteins offer potential biomarkers for HFMD diagnosis and severity assessment.
- These findings lay the groundwork for further investigation into HFMD pathogenesis and regulatory networks.
Abstract:
We implemented 2-D DIGE technology on proteins prepared from serum obtained from children with hand, foot and mouth disease (HFMD) and controls, to study the differentially expressed proteins in control and HFMD serum samples. Proteins found to be differentially expressed were identified with matrix-assisted laser desorption/ionization time-of-flight/ time-of-flight mass spectrometry (MALDI-TOF/TOF MS) analysis. We identified 30 proteins from mild HFMD samples and 39 proteins from severe HFMD samples, compared with the normal controls. 25 proteins among them (14 up-regulated and 11 down-regulated proteins) are found in both HFMD groups. Classification analysis and protein-protein interaction map showed that they associate with multiple functional groups, including transporter activity and atalytic activity. These findings build up a comprehensive profile of the HFMD proteome and provide a useful basis for further analysis of the pathogenic mechanism and the regulatory network of HFMD.
