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Using soybean trypsin inhibitor as an external loading control for Western blot analysis of tear proteins:

Jingjing You1, Chris Hodge, Li Wen

  • 1Save Sight Institute, Discipline of Ophthalmology, University of Sydney, GPO Box 4337, Sydney NSW 2001, Australia. youjingjing@yahoo.com

Experimental Eye Research
|April 14, 2012
PubMed
Abstract

No abstract available in PubMed .

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Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.