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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
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Mass spectrometry-based proteomics: existing capabilities and future directions.

Thomas E Angel1, Uma K Aryal, Shawna M Hengel

  • 1Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA.

Chemical Society Reviews
|April 14, 2012
PubMed
Summary

Mass spectrometry (MS)-based proteomics enables protein identification and quantification for biological understanding. Advanced MS techniques offer sensitive measurements for diverse applications in health and environmental science.

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Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Proteomics is crucial for understanding life's fundamental biological processes.
  • Analyzing proteins at proteome and sub-proteome levels is key to biological insights.
  • Mass spectrometry (MS) is a powerful tool for protein characterization.

Purpose of the Study:

  • To highlight the role of MS-based proteomics in biological research.
  • To discuss emerging technologies enhancing proteomic analysis.
  • To underscore the broad applicability of proteomics in various scientific fields.

Main Methods:

  • Utilizing mass spectrometry (MS) for protein identification, characterization, and quantification.
  • Employing advanced separation techniques like ion mobility, nanoscale reversed-phase liquid chromatography, and capillary electrophoresis.
  • Integrating microchip-based proteome measurements with MS instrumentation.

Main Results:

  • MS-based proteomics provides a foundation for understanding biological systems.
  • Emerging technologies promise enhanced sensitivity and breadth in proteomic measurements.
  • Proteomic data advances the understanding of protein dynamics, interactions, and functions.

Conclusions:

  • MS-based proteomics is a vital and evolving field for biological discovery.
  • Technological advancements are expanding the capabilities of proteomic analysis.
  • Proteomics has wide-ranging applications from microbial studies to human health.